NMDAR1 Recombinant Rabbit Monoclonal Antibody [JM11-26] - BSA and Azide free
Catalog# HA750391
NMDAR1 Recombinant Rabbit Monoclonal Antibody [JM11-26] - BSA and Azide free
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WB
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IF-Tissue
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IHC-P
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IHC-Fr
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Human
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Mouse
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Rat
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Cynomolgus monkey
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Pig
Predicted species support after-sales service
Overview
Product Name
NMDAR1 Recombinant Rabbit Monoclonal Antibody [JM11-26] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human NMDAR1 aa 870-910.
Species Reactivity
Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
Predicted species support after-sales service
Validated Applications
WB, IF-Tissue, IHC-P, IHC-Fr
Molecular Weight
Predicted band size: 105 kDa
Positive Control
MCF7 cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, N2A, SHG-44, SH-SY5Y, mouse cerebral cortex tissue, rat cerebral cortex tissue, mouse hippocampus tissue, mouse cerebral cortex tissue, rat cerebral cortex tissue.
Conjugation
unconjugated
Clone Number
JM11-26
Product Features
Form
Liquid
Concentration
1ug/ul
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:5,000
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IF-Tissue
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1:200-1:500
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IHC-P
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1:1,000
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IHC-Fr
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1:500
Target
Function
Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg2+.Sensitivity to glutamate and channel kinetics depend on the subunit composition.
Background References
1. Zhang X et al. A lasting effect of postnatal sevoflurane anesthesia on the composition of NMDA receptor subunits in rat prefrontal cortex. Int J Dev Neurosci 54:62-69 (2016).
2. Sloniecka M et al. Expression Profiles of Neuropeptides, Neurotransmitters, and Their Receptors in Human Keratocytes In Vitro and In Situ. PLoS One 10:e0134157 (2015).
Sequence Similarity
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily.
Post-translational Modification
NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity.
Subcellular Location
Cell membrane, postsynaptic cell membrane, postsynaptic density.
Synonyms
GluN1 antibody
Glutamate [NMDA] receptor subunit zeta-1 antibody
Glutamate receptor ionotropic N methyl D aspartate 1 antibody
Glutamate receptor ionotropic, N-methyl-D aspartate, subunit 1 antibody
glutamate receptor ionotropic, NMDA 1 antibody
Grin1 antibody
MRD8 antibody
N methyl D aspartate receptor antibody
N methyl D aspartate receptor channel subunit zeta 1 antibody
N methyl D aspartate receptor subunit NR1 antibody
ExpandImages
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Application: IHC-Fr
Species: Mouse
Site: Hippocampus
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Not required -
Application: IHC-Fr
Species: Mouse
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Not required -
Application: IHC-Fr
Species: Rat
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Not required -
Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue with Rabbit anti-NMDAR1 antibody (HA750391) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750391) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue with Rabbit anti-NMDAR1 antibody (HA750391) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750391) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Western blot analysis of NMDAR1 on different lysates with Rabbit anti-NMDAR1 antibody (HA750391) at 1/5,000 dilution.
Lane 1: MCF7 cell lysate (15 µg/Lane)
Lane 2: Human brain tissue lysate (20 µg/Lane)
Lane 3: Mouse brain tissue lysate (20 µg/Lane)
Lane 4: Rat brain tissue lysate (20 µg/Lane)
Lane 5: Mouse heart tissue lysate (negative) (20 µg/Lane)
Lane 6: Rat liver tissue lysate (negative) (20 µg/Lane)
Predicted band size: 105 kDa
Observed band size: 120 kDa
Exposure time: 1 minute 2 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750391) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Application: IF-tissue
Species: Mouse
Site: Cerebral cortex
Sample: Paraffin-embedded section
Antibody concentration: 1:200
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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