beta Amyloid 1-42 Recombinant Rabbit Monoclonal Antibody [PSH02-83] - BSA and Azide free
Catalog# HA750790
beta Amyloid 1-42 Recombinant Rabbit Monoclonal Antibody [PSH02-83] - BSA and Azide free
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WB
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IHC-P
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IF-Tissue
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Human
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Mouse
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HA721789
含抗保成分
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Cynomolgus monkey
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Pig
Predicted species support after-sales service
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unconjugated
Overview
Product Name
beta Amyloid 1-42 Recombinant Rabbit Monoclonal Antibody [PSH02-83] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human beta Amyloid 1-42 peptide.
Species Reactivity
Human, Mouse (Predicted: Cynomolgus monkey, Pig)
Predicted species support after-sales service
Validated Applications
WB, IHC-P, IF-Tissue
Target Molecular Weight
Predicted band size: 4 kDa
Positive Control
APP/PS1, 6-month mouse of AD brain tissue.
Conjugation
unconjugated
Clone Number
PSH02-83
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. In addition, two of the peptides are antimicrobial peptides, having been shown to have bacteriocidal and antifungal activities. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene.
Background References
1. Janelidze S et al. Head-to-Head Comparison of 8 Plasma Amyloid-β 42/40 Assays in Alzheimer Disease. JAMA Neurol. 2021 Nov
Subcellular Location
Cell membrane, Membrane, Perikaryon, Cell projection, growth cone, Membrane, clathrin-coated pit, Early endosome, Cytoplasmic vesicle.
Synonyms
A4 antibody
A4_HUMAN antibody
AAA antibody
ABETA antibody
ABPP antibody
AICD-50 antibody
AICD-57 antibody
AICD-59 antibody
AID(50) antibody
AID(57) antibody
ExpandImages
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This data was developed using HA721789, the same antibody clone in a different buffer formulation.
Western blot analysis of beta Amyloid 1-42 on different peptides with Rabbit anti-beta Amyloid 1-42 antibody (HA721789) at 1/1,000 dilution.
Lane 1: Human Aβ37 full length peptide (negative)
Lane 2: Human Aβ38 full length peptide (negative)
Lane 3: Human Aβ39 full length peptide (negative)
Lane 4: Human Aβ40 full length peptide (negative)
Lane 5: Human Aβ41 full length peptide (negative)
Lane 6: Human Aβ42 full length peptide (positive)
Lane 7: Human Aβ43 full length peptide (negative)
Lane 8: Mouse Aβ42 full length peptide (positive)
Lysates/proteins at 100 ng/Lane.
Predicted band size: 4 kDa
Observed band size: 4 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721789) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA721789, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded APP/PS1, 6-month mouse of AD brain tissue with Rabbit anti-beta Amyloid 1-42 antibody (HA721789) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721789) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
This data was developed using HA721789, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue (negative control) with Rabbit anti-beta Amyloid 1-42 antibody (HA721789) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721789) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA721789, the same antibody clone in a different buffer formulation.
Immunofluorescence analysis of paraffin-embedded APP/PS1, 6-month mouse of AD brain tissue labeling beta Amyloid 1-42 with Rabbit anti-beta Amyloid 1-42 antibody (HA721789) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721789, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"