Neurogranin Recombinant Rabbit Monoclonal Antibody [PSH02-96] - BSA and Azide free
Catalog# HA750851
Neurogranin Recombinant Rabbit Monoclonal Antibody [PSH02-96] - BSA and Azide free
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WB
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IHC-P
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IF-Tissue
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IHC-Fr
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Human
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Mouse
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Rat
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HA721915
含抗保成分
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Cynomolgus monkey
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Pig
Predicted species support after-sales service
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unconjugated
Overview
Product Name
Neurogranin Recombinant Rabbit Monoclonal Antibody [PSH02-96] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human Neurogranin aa 1-78 / 78.
Species Reactivity
Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
Predicted species support after-sales service
Validated Applications
WB, IHC-P, IF-Tissue, IHC-Fr
Target Molecular Weight
Predicted band size: 8 kDa
Positive Control
Mouse brain tissue lysate, mouse hippocampus tissue lysate, rat brain tissue lysate, rat hippocampus tissue lysate, rat brain tissue, mouse brain tissue, mouse hippocampus tissue.
Conjugation
unconjugated
Clone Number
PSH02-96
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Neurogranin is a calmodulin-binding protein expressed primarily in the brain, particularly in dendritic spines, and participating in the protein kinase C signaling pathway. Neurogranin has recently been found in aortic endothelial cells and cardiomyocytes. Neurogranin is the main postsynaptic protein regulating the availability of calmodulin, binding to it in the absence of calcium. Phosphorylation by protein kinase C lowers its binding ability. NRGN gene expression is controlled by thyroid hormones. Human neurogranin consists of 78 amino acids. One study tells of potential link of neurogranin gene to the heightened risk of schizophrenia in males, another study gives evidence of lowered neurogranin immunoreactivity in the brains of people suffering from schizophrenia. Neurogranin concentration in cerebrospinal fluid (CSF) is further discussed as marker for synaptic dysfunction in age-related neurodegeneration. It has also been shown to be specifically increased in patients with Alzheimer's disease. Especially, the ratio of CSF neurogranin trunc P75 and the beta-secretase BACE1 is suggested as a potential marker for cognitive deterioration in the progress of Alzheimer's disease.
Background References
1. Xiang Y et al. Neurogranin: A Potential Biomarker of Neurological and Mental Diseases. Front Aging Neurosci. 2020 Oct
2. Jorgensen AN et al. Neurogranin regulates calcium-dependent cardiac hypertrophy. Exp Mol Pathol. 2022 Aug
Subcellular Location
Axon, cytosol, dendritic spine head, glutamatergic synapse, mitochondrial membrane, neuronal cell body, postsynaptic membrane, trans-Golgi network transport vesicle membrane.
Synonyms
Protein kinase C substrate RC3 antibody
Calmodulin binding protein antibody
Hng antibody
NEUG(55-78) antibody
NEUG_HUMAN antibody
Neurogranin (protein kinase C substrate) antibody
Ng antibody
NRGN antibody
Protein kinase C substrate antibody
RC3 antibody
Images
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This data was developed using HA721915, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Mouse
Site: Hippocampus
Sample: Frozen section
Antibody concentration: 1:200
Antigen retrieval: Recommend. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. -
This data was developed using HA721915, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Mouse
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1:200
Antigen retrieval: Recommend. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. -
This data was developed using HA721915, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Rat
Site: Hippocampus
Sample: Frozen section
Antibody concentration: 1:200
Antigen retrieval: Recommend. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. -
This data was developed using HA721915, the same antibody clone in a different buffer formulation.
Immunofluorescence analysis of frozen rat brain tissue with Rabbit anti-Neurogranin antibody (HA721915) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721915, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
This data was developed using HA721915, the same antibody clone in a different buffer formulation.
Western blot analysis of Neurogranin on different lysates with Rabbit anti-Neurogranin antibody (HA721915) at 1/2,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Mouse hippocampus tissue lysate
Lane 3: Rat brain tissue lysate
Lane 4: Rat hippocampus tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 8 kDa
Observed band size: 15 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721915) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA721915, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Neurogranin antibody (HA721915) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721915) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA721915, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Neurogranin antibody (HA721915) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721915) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA721915, the same antibody clone in a different buffer formulation.
Application: IF-tissue
Species: Mouse
Site: Cerebral cortex
Sample: Paraffin-embedded section
Antibody concentration: 1:200 -
This data was developed using HA721915, the same antibody clone in a different buffer formulation.
Application: IF-tissue
Species: Rat
Site: Cerebral cortex
Sample: Paraffin-embedded section
Antibody concentration: 1:200
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"