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NLRP3 Recombinant Rabbit Monoclonal Antibody [PSH19-43] - BSA and Azide free

Specification

Quota

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Catalog# HA751713

NLRP3 Recombinant Rabbit Monoclonal Antibody [PSH19-43] - BSA and Azide free

Application

  • WB

Reactivity

  • Human

  • Mouse

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

NLRP3 Recombinant Rabbit Monoclonal Antibody [PSH19-43] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human NLRP3 aa 1-250.

Species Reactivity

Human, Mouse

Validated Applications

WB

Molecular Weight

Predicted band size: 118 kDa

Positive Control

THP-1 cell lysate, THP-1 treated with 100ng/mL LPS for 48 hours cell lysate, RAW264.7 cell lysate, J774A.1 cell lysate, RAW264.7 treated with 10μg/mL LPS for 8 hours cell lysate.

Conjugation

unconjugated

Clone Number

PSH19-43

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

Target

Function

NLR family pyrin domain containing 3 (NLRP3) (previously known as NACHT, LRR, and PYD domains-containing protein 3 [NALP3] and cryopyrin), is a protein that in humans is encoded by the NLRP3 gene located on the long arm of chromosome 1. NLRP3 is expressed predominantly in macrophages and as a component of the inflammasome, detects products of damaged cells such as extracellular ATP and crystalline uric acid. Activated NLRP3 in turn triggers an immune response. Mutations in the NLRP3 gene are associated with a number of organ specific autoimmune diseases.

Background References

1. Yu T et al. NLRP3 Cys126 palmitoylation by ZDHHC7 promotes inflammasome activation. Cell Rep. 2024 Apr

2. Tengesdal IW et al. NLRP3 and cancer: Pathogenesis and therapeutic opportunities. Pharmacol Ther. 2023 Nov

Subcellular Location

Cytoplasm, Inflammasome, Secreted, Nucleus, Endoplasmic reticulum.

UNIPROT

SwissProt: Q96P20 Human

SwissProt: Q8R4B8 Mouse

Synonyms

AGTAVPRL antibody

AII/AVP antibody

Angiotensin/vasopressin receptor AII/AVP like antibody

Angiotensin/vasopressin receptor AII/AVP-like antibody

C1orf7 antibody

Caterpiller protein 1.1 antibody

CIAS 1 antibody

CIAS1 antibody

CLR1.1 antibody

Cold autoinflammatory syndrome 1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of NLRP3 on different lysates with Rabbit anti-NLRP3 antibody (<a href="/products/HA751713" style="font-weight: bold;text-decoration: underline;">HA751713</a>) at 1/5,000 dilution.<br /><br />Lane 1: THP-1 cell lysate<br />Lane 2: THP-1 treated with 100ng/mL LPS for 48 hours cell lysate<br />Lane 3: Jurkat cell lysate (low expression)<br />Lane 4: RAW264.7 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 118 kDa<br />Observed band size: 118 kDa<br /><br />Exposure time: 1 minute 50 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751713" style="font-weight: bold;text-decoration: underline;">HA751713</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of NLRP3 on different lysates with Rabbit anti-NLRP3 antibody (HA751713) at 1/5,000 dilution.

    Lane 1: THP-1 cell lysate
    Lane 2: THP-1 treated with 100ng/mL LPS for 48 hours cell lysate
    Lane 3: Jurkat cell lysate (low expression)
    Lane 4: RAW264.7 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 118 kDa
    Observed band size: 118 kDa

    Exposure time: 1 minute 50 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751713) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of NLRP3 on different lysates with Rabbit anti-NLRP3 antibody (<a href="/products/HA751713" style="font-weight: bold;text-decoration: underline;">HA751713</a>) at 1/5,000 dilution.<br /><br />Lane 1: J774A.1 cell lysate<br />Lane 2: NIH/3T3 cell lysate (low expression)<br />Lane 3: RAW264.7 cell lysate<br />Lane 4: RAW264.7 treated with 10μg/mL LPS for 8 hours cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 118 kDa<br />Observed band size: 118 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751713" style="font-weight: bold;text-decoration: underline;">HA751713</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of NLRP3 on different lysates with Rabbit anti-NLRP3 antibody (HA751713) at 1/5,000 dilution.

    Lane 1: J774A.1 cell lysate
    Lane 2: NIH/3T3 cell lysate (low expression)
    Lane 3: RAW264.7 cell lysate
    Lane 4: RAW264.7 treated with 10μg/mL LPS for 8 hours cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 118 kDa
    Observed band size: 118 kDa

    Exposure time: 25 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751713) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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