GABA B Receptor 2 Recombinant Rabbit Monoclonal Antibody [JU31-32] - BSA and Azide free
Catalog# HA751832
GABA B Receptor 2 Recombinant Rabbit Monoclonal Antibody [JU31-32] - BSA and Azide free
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WB
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IHC-P
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IHC-Fr
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IF-Tissue
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Human
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Mouse
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Rat
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ET7106-88
含抗保成分
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Cynomolgus monkey
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Pig
Predicted species support after-sales service
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unconjugated
Overview
Product Name
GABA B Receptor 2 Recombinant Rabbit Monoclonal Antibody [JU31-32] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within C-terminal Human GABA B Receptor 2 .
Species Reactivity
Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
Predicted species support after-sales service
Validated Applications
WB, IHC-P, IHC-Fr, IF-Tissue
Target Molecular Weight
Predicted band size: 106 kDa
Positive Control
Mouse brain tissue lysate, rat brain tissue lysate, mouse brain tissue, rat brain tissue, mouse cerebellum tissue, SH-SY-5Y.
Conjugation
unconjugated
Clone Number
JU31-32
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
In the central nervous system (CNS), gamma-aminobutyric acid (GABA) is the main main inhibitory neurotransmitter that functions to regulate neuronal firing. GABA exerts its effects through two different kinds of receptors: ionotropic receptors (GABAA R and GABAC R), which produce fast inhibitory signals, and metabotropic receptors (GABAB R), which produce slow inhibitory signals. The GABAB R receptor is a heterodimer that consists of two multi-pass membrane proteins, designated GABAB R1 and GABAB R2, both of which belong to the G protein-coupled receptor family and are highly expressed in brain tissue. Together, GABAB R1 and GABAB R2 play a crucial role in the fine-tuning of inhibitory synaptic transmissions and are implicated in slow wave sleep, muscle relaxation, hippocampal long-term potentiation and antinociception events. Both GABAB R1 and GABAB R2 are regulated by G proteins that have a variety of functions, including activation of potassium channels, inhibition of adenylyl cyclase (A cyclase) activity and modulation of inositol phospholipid hydrolysis.
Background References
1. White J H et al. Heterodimerization is required for the formation of a functional GABA(B) receptor. Nature 396:679-682 (1998).
2. Martin S C et al. Molecular identification of the human GABABR2: cell surface expression and coupling to adenylyl cyclase in the absence of GABABR1. Mol Cell Neurosci 13:180-191 (1999).
Sequence Similarity
Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.
Subcellular Location
Plasma membrane.
Synonyms
BcDNA:GH07312 antibody
CG6706 antibody
CT20836 antibody
D Gaba2 antibody
FLJ36928 antibody
G protein coupled receptor 51 antibody
G-protein coupled receptor 51 antibody
GAB B R2 antibody
GABA B R2 antibody
GABA B receptor 2 antibody
ExpandImages
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This data was developed using ET7106-88, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Mouse
Site: Cerebellum
Sample: Frozen section
Antibody concentration: 1:1,000
Antigen retrieval: Not required -
This data was developed using ET7106-88, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Rat
Site: Cerebellum
Sample: Frozen section
Antibody concentration: 1:1,000
Antigen retrieval: Not required -
This data was developed using ET7106-88, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Mouse
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Not required -
This data was developed using ET7106-88, the same antibody clone in a different buffer formulation.
Application: IF-tissue
Species: Mouse
Site: Cerebral cortex
Sample: Paraffin-embedded section
Antibody concentration: 1:500 -
This data was developed using ET7106-88, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-GABA B Receptor 2 antibody (ET7106-88) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-88) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET7106-88, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-GABA B Receptor 2 antibody (ET7106-88) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-88) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET7106-88, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-GABA B Receptor 2 antibody (ET7106-88) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-88) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET7106-88, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-GABA B Receptor 2 antibody (ET7106-88) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-88) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET7106-88, the same antibody clone in a different buffer formulation.
Western blot analysis of GABA B Receptor 2 on different lysates with Rabbit anti-GABA B Receptor 2 antibody (ET7106-88) at 1/1,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Mouse brain tissue lysate (no heat)
Lane 3: Rat brain tissue lysate
Lane 4: Rat brain tissue lysate (no heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 106 kDa
Observed band size: 106 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-88) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"