Synaptophysin Recombinant Rabbit Monoclonal Antibody [SJ26-85] - BSA and Azide free
Catalog# HA751835
Synaptophysin Recombinant Rabbit Monoclonal Antibody [SJ26-85] - BSA and Azide free
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WB
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IHC-P
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IHC-Fr
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IF-Tissue
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Human
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Mouse
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Rat
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ET1606-56
含抗保成分
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Cynomolgus monkey
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Pig
Predicted species support after-sales service
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unconjugated
Overview
Product Name
Synaptophysin Recombinant Rabbit Monoclonal Antibody [SJ26-85] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Synaptophysin aa 224 – 313 (Cytoplasmic).
Species Reactivity
Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
Predicted species support after-sales service
Validated Applications
WB, IHC-P, IHC-Fr, IF-Tissue
Target Molecular Weight
Predicted band size: 34 kDa
Positive Control
SH-SY5Y cell lysate, PC-12 cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, atypical carcinoid tissue, human medullary thyroid carcinoma tissue, human pancreas tissue, human small intestine tissue, mouse cerebellum tissue, mouse pancreas tissue, rat cerebellum tissue.
Conjugation
unconjugated
Clone Number
SJ26-85
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Synaptic vesicles participate in a cycle of fusion with the plasma membrane and reformation by endocytosis. Synaptic vesicle protein synaptophysin (SYP) is targeted to early endosomes in transfected fibroblasts and in neuroendocrine cells. SYP is an N-glycosylated intergral membrane protein found in neurons and endocrine cells that associates into hexamers to form a large conductance channel. SYP contains four transmembrane domains and may function as a gap juction-like channel. Membrane cholesterol specfically interacts with SYP to play a role in vesicle formation. Synaptobrevin (VAMP) also binds to SYP and the resultant complex is upregulated during neuronal development, but is absent in exocytosis fusion complex. Thus, the synaptophysin-synaptobrevin complex is not essential for exocytosis, but rather provides a pool of synaptobrevin for exocytosis. In addition, the tail domain of brain Myosin V also forms a stable complex with synaptobrevin II and SYP, and this complex is disassembled upon the depolarization-induced entry of Ca2+ into intact nerve endings.
Background References
1. Atzpodien EA et al. Advanced Clinical Imaging and Tissue-based Biomarkers of the Eye for Toxicology Studies in Minipigs. Toxicol Pathol 44:398-413 (2016).
2. Ren M et al. A biofidelic 3D culture model to study the development of brain cellular systems. Sci Rep 6:24953 (2016).
Sequence Similarity
Belongs to the synaptophysin/synaptobrevin family.
Tissue Specificity
Expressed in the brain, with expression in the hippocampus, the neuropil in the dentate gyrus, where expression is higher in the outer half of the molecular layer than in the inner half, and in the neuropil of CA4 and CA3.
Post-translational Modification
Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
Subcellular Location
Cytoplasmic, Cell junction, synapse, synaptosome.
Synonyms
Major synaptic vesicle protein p38 antibody
MRX96 antibody
MRXSYP antibody
Syn p38 antibody
Synaptophysin antibody
Syp antibody
SYPH antibody
SYPH_HUMAN antibody
SypI antibody
Images
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This data was developed using ET1606-56, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Mouse
Site: Cerebellum
Sample: Frozen section
Antibody concentration: 1/1,000
Antigen retrieval: Not required -
This data was developed using ET1606-56, the same antibody clone in a different buffer formulation.
Application: IF-tissue
Species: Mouse
Site: Pancreas
Sample: Paraffin-embedded section
Antibody concentration: 1/500 -
This data was developed using ET1606-56, the same antibody clone in a different buffer formulation.
Application: IF-tissue
Species: Mouse
Site: Cerebellum
Sample: Paraffin-embedded section
Antibody concentration: 1/500 -
☑ Relative expression (RE)
This data was developed using ET1606-56, the same antibody clone in a different buffer formulation.
Western blot analysis of Synaptophysin on different lysates with Rabbit anti-Synaptophysin antibody (ET1606-56) at 1/5,000 dilution.
Lane 1: SH-SY5Y cell lysate
Lane 2: HeLa cell lysate (negative)
Lane 3: NIH/3T3 cell lysate (negative)
Lane 4: PC-12 cell lysate
Lane 5: Human brain tissue lysate
Lane 6: Mouse brain tissue lysate
Lane 7: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 34 kDa
Observed band size: 34/40 kDa
Exposure time: 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-56) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. -
This data was developed using ET1606-56, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded atypical carcinoid tissue with Rabbit anti-Synaptophysin antibody (ET1606-56) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-56) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1606-56, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human medullary thyroid carcinoma tissue with Rabbit anti-Synaptophysin antibody (ET1606-56) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-56) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1606-56, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Synaptophysin antibody (ET1606-56) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-56) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1606-56, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Synaptophysin antibody (ET1606-56) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-56) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1606-56, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-Synaptophysin antibody (ET1606-56) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-56) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1606-56, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-Synaptophysin antibody (ET1606-56) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-56) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1606-56, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-Synaptophysin antibody (ET1606-56) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-56) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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