IL-6 Recombinant Rabbit Monoclonal Antibody
Catalog# IRS160RB
IL-6 Recombinant Rabbit Monoclonal Antibody
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mIHC
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Human
Overview
Product Name
IL-6 Recombinant Rabbit Monoclonal Antibody
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein (or fragment).
Species Reactivity
Human
Validated Applications
mIHC
Molecular Weight
Predicted band size: 24 kDa
Positive Control
Human tonsil tissue, human lung tissue, human intrahepatic cholangiocarcinoma tissue, human appendix tissue, human gastric cancer tissue, human pancreatic cancer tissue.
Conjugation
unconjugated
Product Features
Form
Liquid
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Affinity purification
Application Dilution
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mIHC
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1:100
Target
Function
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ). An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Background References
1. Timper K., Denson J.L., Steculorum S.M., Heilinger C., Engstroem-Ruud L., Wunderlich C.M., Rose-John S., Wunderlich F.T., Bruening J.C. IL-6 Improves Energy and Glucose Homeostasis in Obesity via Enhanced Central IL-6 trans-Signaling. Cell Rep. 19:267-280 (2017)
2. Ivanov I.I., McKenzie B.S., Zhou L., Tadokoro C.E., Lepelley A., Lafaille J.J., Cua D.J., Littman D.R. The orphan nuclear receptor RORgammat directs the differentiation program of proinflammatory IL-17+ T helper cells. Cell 126:1121-1133 (2006)
Subcellular Location
Secreted.
UNIPROT
Synonyms
Interleukin BSF 2 antibody
B cell differentiation factor antibody
B cell stimulatory factor 2 antibody
B-cell stimulatory factor 2 antibody
BSF 2 antibody
BSF-2 antibody
BSF2 antibody
CDF antibody
CTL differentiation factor antibody
Cytotoxic T cell differentiation factor antibody
ExpandImages
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mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-IL-6 antibody (IRS160RB) at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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mIHC analysis of human lung tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-IL-6 antibody (IRS160RB) at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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mIHC analysis of human intrahepatic cholangiocarcinoma tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-IL-6 antibody (IRS160RB) at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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mIHC analysis of human appendix tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-IL-6 antibody (IRS160RB) at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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mIHC analysis of human gastric cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-IL-6 antibody (IRS160RB) at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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mIHC analysis of human pancreatic cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-IL-6 antibody (IRS160RB) at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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