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CD68 Recombinant Mouse Monoclonal Antibody

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Catalog# IRS004

CD68 Recombinant Mouse Monoclonal Antibody

Application

  • mIHC

Reactivity

  • Human

Overview

Product Name

CD68 Recombinant Mouse Monoclonal Antibody

Antibody Type

Recombinant Mouse Monoclonal Antibody

Immunogen

Recombinant protein within Human CD68 aa 1-354.

Species Reactivity

Human

Validated Applications

mIHC

Positive Control

Human tonsil tissue

Conjugation

unconjugated

Product Features

Form

Liquid

Concentration

1ug/ul

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • mIHC

  • 1:100. This antibody has been validated for multiplex staining with LUMINIRIS mTSA, supporting both multiplex and super-multiplex staining.

Target

Function

CD68 (Cluster of Differentiation 68) is a protein highly expressed by cells in the monocyte lineage (e.g., monocytic phagocytes, osteoclasts), by circulating macrophages, and by tissue macrophages (e.g., Kupffer cells, microglia). Human CD68 is a transmembrane glycoprotein, heavily glycosylated in its extracellular domain, with a molecular weight of 110 kD. Its primary sequence consists of 354 amino acids with predicted molecular weight of 37.4 kD if it were not glycosylated. Immunohistochemistry can be used to identify the presence of CD68, which is found in the cytoplasmic granules of a range of different blood cells and myocytes. It is particularly useful as a marker for the various cells of the macrophage lineage, including monocytes, histiocytes, giant cells, Kupffer cells, and osteoclasts. This allows it to be used to distinguish diseases of otherwise similar appearance, such as the monocyte/macrophage and lymphoid forms of leukaemia (the latter being CD68 negative). Its presence in macrophages also makes it useful in diagnosing conditions related to proliferation or abnormality of these cells, such as malignant histiocytosis, histiocytic lymphoma, and Gaucher's disease.

Background References

1. Wang L. et. al. Specific clinical and immune features of CD68 in glioma via 1,024 samples. Cancer Manag Res. 2018 Nov 27;10:6409-6419.

2. Minami K. et. al. Prognostic significance of CD68, CD163 and Folate receptor-β positive macrophages in hepatocellular carcinoma. Exp Ther Med. 2018 May;15(5):4465-4476.

Subcellular Location

Cell membrane. Endosome membrane, Lysosome membrane.

UNIPROT

SwissProt: P34810 Human

Synonyms

CD 68 antibody

CD68 antibody

CD68 antigen antibody

CD68 molecule antibody

CD68_HUMAN antibody

DKFZp686M18236 antibody

gp11 antibody

Gp110 antibody

LAMP4 antibody

Macrophage antigen CD68 (microsialin) antibody

Expand

Images

  • mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Mouse anti-CD68 antibody (<a href="/products/IRS004" style="font-weight: bold;text-decoration: underline;">IRS004</a>) at 1/100 dilution. The immunostaining was performed with the IRISKit&reg; HyperView mTSA Kit (<a href="/products/MH900206" style="font-weight: bold;text-decoration: underline;">MH900206</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Mouse anti-CD68 antibody (IRS004) at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human cholangiocarcinoma tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD11c (<a href="/products/IRS001" style="font-weight: bold;text-decoration: underline;">IRS001</a>), CD68 (<a href="/products/IRS004" style="font-weight: bold;text-decoration: underline;">IRS004</a>), CD20 (<a href="/products/IRS003" style="font-weight: bold;text-decoration: underline;">IRS003</a>), CD38 (<a href="/products/IRS006" style="font-weight: bold;text-decoration: underline;">IRS006</a>), CD8 and panCK (<a href="/products/IRS010" style="font-weight: bold;text-decoration: underline;">IRS010</a>) antibody at 1/100 dilution. The immunostaining was performed with the IRISKit&reg; HyperView mTSA Kit (<a href="/products/MH900206" style="font-weight: bold;text-decoration: underline;">MH900206</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human cholangiocarcinoma tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD11c (IRS001), CD68 (IRS004), CD20 (IRS003), CD38 (IRS006), CD8 and panCK (IRS010) antibody at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD8, CD4 (<a href="/products/IRS005" style="font-weight: bold;text-decoration: underline;">IRS005</a>), FOXP3, CD68 (<a href="/products/IRS004" style="font-weight: bold;text-decoration: underline;">IRS004</a>), CD38 (<a href="/products/IRS006" style="font-weight: bold;text-decoration: underline;">IRS006</a>), CD11c (<a href="/products/IRS001" style="font-weight: bold;text-decoration: underline;">IRS001</a>), PD-L1, CD163 (<a href="/products/IRS002" style="font-weight: bold;text-decoration: underline;">IRS002</a>) and CD20 (<a href="/products/IRS003" style="font-weight: bold;text-decoration: underline;">IRS003</a>) antibody at 1/100 dilution. The immunostaining was performed with the IRISKit&reg; HyperView mTSA Kit (<a href="/products/MH900206" style="font-weight: bold;text-decoration: underline;">MH900206</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD8, CD4 (IRS005), FOXP3, CD68 (IRS004), CD38 (IRS006), CD11c (IRS001), PD-L1, CD163 (IRS002) and CD20 (IRS003) antibody at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human lung cancer with brain metastasis tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD3, CD20 (<a href="/products/IRS003" style="font-weight: bold;text-decoration: underline;">IRS003</a>), panCK (<a href="/products/IRS010" style="font-weight: bold;text-decoration: underline;">IRS010</a>), CD68 (<a href="/products/IRS004" style="font-weight: bold;text-decoration: underline;">IRS004</a>) and Ki67 antibody at 1/100 dilution. The immunostaining was performed with the IRISKit&reg; HyperView mTSA Kit (<a href="/products/MH900206" style="font-weight: bold;text-decoration: underline;">MH900206</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human lung cancer with brain metastasis tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD3, CD20 (IRS003), panCK (IRS010), CD68 (IRS004) and Ki67 antibody at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human cervical cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD8, CD20 (<a href="/products/IRS003" style="font-weight: bold;text-decoration: underline;">IRS003</a>), FOXP3, CK19 (<a href="/products/IRS011" style="font-weight: bold;text-decoration: underline;">IRS011</a>), PD-L1, CD4 (<a href="/products/IRS005" style="font-weight: bold;text-decoration: underline;">IRS005</a>), CD163 (<a href="/products/IRS002" style="font-weight: bold;text-decoration: underline;">IRS002</a>) and CD68 (<a href="/products/IRS004" style="font-weight: bold;text-decoration: underline;">IRS004</a>) antibody at 1/100 dilution. The immunostaining was performed with the IRISKit&reg; HyperView mTSA Kit (<a href="/products/MH900206" style="font-weight: bold;text-decoration: underline;">MH900206</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human cervical cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD8, CD20 (IRS003), FOXP3, CK19 (IRS011), PD-L1, CD4 (IRS005), CD163 (IRS002) and CD68 (IRS004) antibody at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human colorectal cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD8, CD20 (<a href="/products/IRS003" style="font-weight: bold;text-decoration: underline;">IRS003</a>), CD68 (<a href="/products/IRS004" style="font-weight: bold;text-decoration: underline;">IRS004</a>), CD4 (<a href="/products/IRS005" style="font-weight: bold;text-decoration: underline;">IRS005</a>) and panCK (<a href="/products/IRS010" style="font-weight: bold;text-decoration: underline;">IRS010</a>) antibody at 1/100 dilution. The immunostaining was performed with the IRISKit&reg; HyperView mTSA Kit (<a href="/products/MH900206" style="font-weight: bold;text-decoration: underline;">MH900206</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human colorectal cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD8, CD20 (IRS003), CD68 (IRS004), CD4 (IRS005) and panCK (IRS010) antibody at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human gastric cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with PD-L1, CD8, CD3, CD68 (<a href="/products/IRS004" style="font-weight: bold;text-decoration: underline;">IRS004</a>), CD20 (<a href="/products/IRS003" style="font-weight: bold;text-decoration: underline;">IRS003</a>) and panCK (<a href="/products/IRS010" style="font-weight: bold;text-decoration: underline;">IRS010</a>) antibody at 1/100 dilution. The immunostaining was performed with the IRISKit&reg; HyperView mTSA Kit (<a href="/products/MH900206" style="font-weight: bold;text-decoration: underline;">MH900206</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human gastric cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with PD-L1, CD8, CD3, CD68 (IRS004), CD20 (IRS003) and panCK (IRS010) antibody at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human small cell lung cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with PD-L1, CD8, CD3, CD68 (<a href="/products/IRS004" style="font-weight: bold;text-decoration: underline;">IRS004</a>), CD20 (<a href="/products/IRS003" style="font-weight: bold;text-decoration: underline;">IRS003</a>) and panCK (<a href="/products/IRS010" style="font-weight: bold;text-decoration: underline;">IRS010</a>) antibody at 1/100 dilution. The immunostaining was performed with the IRISKit&reg; HyperView mTSA Kit (<a href="/products/MH900206" style="font-weight: bold;text-decoration: underline;">MH900206</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human small cell lung cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with PD-L1, CD8, CD3, CD68 (IRS004), CD20 (IRS003) and panCK (IRS010) antibody at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human pancreatic cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD3, CD68 (<a href="/products/IRS004" style="font-weight: bold;text-decoration: underline;">IRS004</a>), CD20 (<a href="/products/IRS003" style="font-weight: bold;text-decoration: underline;">IRS003</a>) and panCK (<a href="/products/IRS010" style="font-weight: bold;text-decoration: underline;">IRS010</a>) antibody at 1/100 dilution. The immunostaining was performed with the IRISKit&reg; HyperView mTSA Kit (<a href="/products/MH900206" style="font-weight: bold;text-decoration: underline;">MH900206</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human pancreatic cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD3, CD68 (IRS004), CD20 (IRS003) and panCK (IRS010) antibody at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human endometrial cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with PD-L1, CD8, CD3, CK19 (<a href="/products/IRS011" style="font-weight: bold;text-decoration: underline;">IRS011</a>), FOXP3 and CD68 (<a href="/products/IRS004" style="font-weight: bold;text-decoration: underline;">IRS004</a>) antibody at 1/100 dilution. The immunostaining was performed with the IRISKit&reg; HyperView mTSA Kit (<a href="/products/MH900206" style="font-weight: bold;text-decoration: underline;">MH900206</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human endometrial cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with PD-L1, CD8, CD3, CK19 (IRS011), FOXP3 and CD68 (IRS004) antibody at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD56 (<a href="/products/IRS026" style="font-weight: bold;text-decoration: underline;">IRS026</a>), CD68 (<a href="/products/IRS004" style="font-weight: bold;text-decoration: underline;">IRS004</a>), CD163 (<a href="/products/IRS002" style="font-weight: bold;text-decoration: underline;">IRS002</a>), panCK (<a href="/products/IRS010" style="font-weight: bold;text-decoration: underline;">IRS010</a>) and CD20 (<a href="/products/IRS003" style="font-weight: bold;text-decoration: underline;">IRS003</a>) antibody at 1/100 dilution. The immunostaining was performed with the IRISKit&reg; HyperView mTSA Kit (<a href="/products/MH900206" style="font-weight: bold;text-decoration: underline;">MH900206</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD56 (IRS026), CD68 (IRS004), CD163 (IRS002), panCK (IRS010) and CD20 (IRS003) antibody at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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