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Ki67 Rabbit Polyclonal Antibody

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Catalog# ER1706-46

Ki67 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Ki67 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within human Ki67 aa 1009-1094.

Species Reactivity

Human

Validated Applications

WB, IF-Cell, IHC-P, FC

Molecular Weight

Predicted band size: 359 kDa

Positive Control

HepG2, A431, A549, LOVO, human tonsil tissue, human lung cancer tissue, human colon cancer tissue, human stomach cancer tissue.

Conjugation

unconjugated

RRID

AB_3069061

Product Features

Form

Liquid

Concentration

1ug/ul

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1,000

  • IF-Cell

  • 1:200-1:1,000

  • IHC-P

  • 1:100-1:500

  • FC

  • 1:50-1:200

Species in Publications

Mouse See 4 publications below
Human See 3 publications below
Dairy cattle See 1 publications below

Target

Function

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. Binds DNA, with a preference for supercoiled DNA and AT-rich DNA. Does not contribute to the internal structure of mitotic chromosomes. May play a role in chromatin organization.

Background References

1. Cuylen S et al. Ki-67 acts as a biological surfactant to disperse mitotic chromosomes. Nature 535:308-312 (2016).

2. Booth DG et al. Ki-67 is a PP1-interacting protein that organises the mitotic chromosome periphery. Elife 3:E01641-E01641 (2014).

Post-translational Modification

Phosphorylated. Hyperphosphorylated in mitosis. Hyperphosphorylated form does not bind DNA.

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P46013 Human

Synonyms

Antigen identified by monoclonal antibody Ki 67 antibody

Antigen identified by monoclonal antibody Ki-67 antibody

Antigen KI-67 antibody

Antigen KI67 antibody

Antigen Ki67 antibody

KI67_HUMAN antibody

KIA antibody

Marker of proliferation Ki-67 antibody

MIB 1 antibody

MIB antibody

Expand

Images

  • ICC staining Ki67 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Ki67 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining Ki67 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Ki67 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining Ki67 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Ki67 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Ki67 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Ki67 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Ki67 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Ki67 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

  • Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Ki67 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Ki67 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Ki67 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Ki67 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

  • Flow cytometric analysis of Hela cells with Ki67 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

    Flow cytometric analysis of Hela cells with Ki67 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

  • Western blot analysis of Ki67 on HepG2 cell lysate using anti-Ki67 antibody at 1/1,000 dilution.

    Western blot analysis of Ki67 on HepG2 cell lysate using anti-Ki67 antibody at 1/1,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Filter:
  1. IHC
  2. IF
  3. WB

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