LC3B Recombinant Antibody [JJ090-6] - Mouse IgG1 (Chimeric)
Catalog# HA601532
LC3B Recombinant Antibody [JJ090-6] - Mouse IgG1 (Chimeric)
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
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Rat
Overview
Product Name
LC3B Recombinant Antibody [JJ090-6] - Mouse IgG1 (Chimeric)
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Synthetic peptide within human LC3 B aa 1-20.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P
Molecular Weight
Predicted band size: 14/16 kDa
Positive Control
HeLa cell lysate, HeLa treated with 50μM Chloroquine for 18 hours cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, HeLa cells treated with 50μM Chloroquine for 18 hours, mouse brain tissue, rat brain tissue.
Conjugation
unconjugated
Clone Number
JJ090-6
Product Features
Form
Liquid
Concentration
1ug/ul
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IF-Cell
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1:200
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IHC-P
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1:2,000
Target
Function
Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and in maintaining the balance between neuronal plasticity and rigidity. MAP-light chain 3 beta (MAP-LC3β) and MAP-light chain 3 alpha (MAP-LC3α) are subunits of both MAP1A and MAP1B. MAP-LC3β, a homolog of Apg8p, is essential for autophagy and associated to the autophagosome membranes after processing. Two forms of LC3β, the cytosolic LC3-I and the membrane-bound LC3-II, are produced post-translationally. LC3-I is formed by the removal of the C-terminal 22 amino acids from newly synthesized LC3β, followed by the conversion of a fraction of LC3-I into LC3-II. LC3 enhances fibronectin mRNA translation in ductus arteriosus cells through association with 60S ribosomes and binding to an AU-rich element in the 3′ untranslated region of fibronectin mRNA. This facilitates sorting of fibronectin mRNA onto rough endoplasmic reticulum and translation. MAP LC3β may also be involved in formation of autophagosomal vacuoles. It is expressed primarily in heart, testis, brain and skeletal muscle.
Background References
1. Omrane M et al. LC3B is lipidated to large lipid droplets during prolonged starvation for noncanonical autophagy. Dev Cell. 2023 Jul
2. Hwang HJ et al. LC3B is an RNA-binding protein to trigger rapid mRNA degradation during autophagy. Nat Commun. 2022 Mar
Subcellular Location
Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Microtubule, Mitochondrion.
Synonyms
ATG8F antibody
Autophagy-related protein LC3 B antibody
Autophagy-related ubiquitin-like modifier LC3 B antibody
LC3B antibody
LC3II antibody
MAP1 light chain 3 like protein 2 antibody
MAP1 light chain 3-like protein 2 antibody
MAP1A/1BLC3 antibody
MAP1A/MAP1B LC3 B antibody
MAP1A/MAP1B light chain 3 B antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of LC3B on different lysates with Mouse anti-LC3B antibody (HA601532) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 50μM Chloroquine for 18 hours cell lysate
Lane 3: Mouse brain tissue lysate
Lane 4: Rat brain tissue lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 14/16 kDa
Observed band size: 14/16 kDa
Exposure time: 42 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601532) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HeLa cells untreated / treated with 50μM Chloroquine for 18 hours labeling LC3B with Mouse anti-LC3B antibody (HA601532) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-LC3B antibody (HA601532) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-LC3B antibody (HA601532) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601532) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-LC3B antibody (HA601532) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601532) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
LC3B Recombinant Rabbit Monoclonal Antibody [JJ090-6] - BSA and Azide free
Application: WB,IF-Cell,IHC-P,IF-Tissue,IP
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
