LRPPRC Recombinant Rabbit Monoclonal Antibody [PSH24-58] - BSA and Azide free
Catalog# HA751948
LRPPRC Recombinant Rabbit Monoclonal Antibody [PSH24-58] - BSA and Azide free
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WB
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IF-Cell
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IHC-P
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FC(Intra)
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Human
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Mouse
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Rat
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Green monkey
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Pig
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HA724454
含抗保成分
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unconjugated
Overview
Product Name
LRPPRC Recombinant Rabbit Monoclonal Antibody [PSH24-58] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human LRPPRC aa 651-870.
Species Reactivity
Human, Mouse, Rat, Green monkey, Pig
Validated Applications
WB, IF-Cell, IHC-P, FC(Intra)
Target Molecular Weight
Predicted band size: 158 kDa
Positive Control
HeLa (Human cervical adenocarcinoma cell) cell lysate, HEK-293 (Human embryonic kidney cell) cell lysate, HepG2 (Human liver cancer cells) cell lysate, SH-SY5Y (Human neuroblastoma cell) cell lysate, RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate, Neuro-2a (Mouse brain neuroblastoma cell) cell lysate, PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate, COS-1 (African green monkey kidney fibroblast) cell lysate, Mouse brain tissue lysate, Mouse heart tissue lysate, Rat brain tissue lysate, Rat heart tissue lysate, Pig brain tissue lysate.
Conjugation
unconjugated
Clone Number
PSH24-58
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | FC(Intra) | IF-Cell | |
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| human |
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| mouse |
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| rat |
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| green monkey |
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| pig |
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Product Features
Form
Liquid
Concentration
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Leucine-rich PPR motif-containing protein, mitochondrial is a protein that in humans is encoded by the LRPPRC gene. Transcripts ranging in size from 4.8 to 7.0 kb which result from alternative polyadenylation have been reported for this gene. This gene encodes a protein that is leucine-rich and is thought to play a role in regulating the interaction of the cytoskeleton with a variety of cellular processes.
Background References
1. Zhou W et al. The RNA-binding protein LRPPRC promotes resistance to CDK4/6 inhibition in lung cancer. Nat Commun. 2023 Jul
2. Rubalcava-Gracia D et al. LRPPRC and SLIRP synergize to maintain sufficient and orderly mammalian mitochondrial translation. Nucleic Acids Res. 2024 Oct
Subcellular Location
Mitochondrion, Nucleus, nucleoplasm, Nucleus inner membrane, Nucleus outer membrane.
Synonyms
Leucine-rich PPR motif-containing protein, mitochondrial
130 kDa leucine-rich protein (LRP 130)
GP130
LRPPRC
LRP130
Images
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This data was developed using HA724454, the same antibody clone in a different buffer formulation.
Western blot analysis of LRPPRC on different lysates with Rabbit anti-LRPPRC antibody (HA724454) at 1/10,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 2: HEK-293 (Human embryonic kidney cell) cell lysate
Lane 3: HepG2 (Human liver cancer cells) cell lysate
Lane 4: SH-SY5Y (Human neuroblastoma cell) cell lysate
Lane 5: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate
Lane 6: NIH/3T3 (Mouse fibroblast) cell lysate
Lane 7: Neuro-2a (Mouse brain neuroblastoma cell) cell lysate
Lane 8: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate
Lane 9: COS-1 (African green monkey kidney fibroblast) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 7 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724454, 1/10,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 158 kDa
Observed band size: 150 kDa -
This data was developed using HA724454, the same antibody clone in a different buffer formulation.
Western blot analysis of LRPPRC on different lysates with Rabbit anti-LRPPRC antibody (HA724454) at 1/10,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Mouse heart tissue lysate
Lane 3: Rat brain tissue lysate
Lane 4: Rat heart tissue lysate
Lane 5: Pig brain tissue lysate
Lysates/proteins at 30 µg/Lane.
Exposure time: 7 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724454, 1/10,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 158 kDa
Observed band size: 150 kDa -
This data was developed using HA724454, the same antibody clone in a different buffer formulation.
Application: Immunocytochemistry (IF-cell)
Species: Human
Sample: HeLa (Human cervix adenocarcinoma epithelial cell)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA724454, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). -
This data was developed using HA724454, the same antibody clone in a different buffer formulation.
Application: Immunocytochemistry (IF-cell)
Species: Mouse
Sample: NIH/3T3 (Mouse fibroblast)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA724454, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). -
This data was developed using HA724454, the same antibody clone in a different buffer formulation.
Application: Immunocytochemistry (IF-cell)
Species: Rat
Sample: PC-12 (Rat pheochromocytoma cell (undifferentiated))
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA724454, 1/100, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). -
This data was developed using HA724454, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Kidney
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724454, 1/50, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
This data was developed using HA724454, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Colon
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724454, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
This data was developed using HA724454, the same antibody clone in a different buffer formulation.
Application: Flow Cytometry (Intra)
Species: Rat
Sample: PC-12 (Rat pheochromocytoma cells (undifferentiated))
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA724454(1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"