Lentivirus HIV-1 p24 enzyme-linked immunoassay kit(one step)
Specification
General Overview
Product Name
Lentivirus HIV-1 p24 enzyme-linked immunoassay kit(one step)
Product Type
Elisa Kit
Sensitivity
0.12 ng/ml (10 μl)

ng/ml | O.D. | Average | Corrected | |
---|---|---|---|---|
0.00 | 0.0072 | 0.0068 | 0.0070 | |
4.12 | 0.0220 | 0.0212 | 0.0216 | 0.0146 |
12.35 | 0.0453 | 0.0461 | 0.0457 | 0.0387 |
37.04 | 0.1085 | 0.1066 | 0.1076 | 0.1006 |
111.11 | 0.2794 | 0.2775 | 0.2785 | 0.2715 |
333.33 | 0.6978 | 0.6874 | 0.6926 | 0.6856 |
1000.00 | 1.7410 | 1.6832 | 1.7121 | 1.7051 |
3000.00 | 2.7224 | 2.6821 | 2.7023 | 2.6953 |
Precision
Intra-assay Precision | Inter-assay Precision | |||||
Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
22 | 22 | 22 | 6 | 6 | 6 | |
Average (ng/ml) | 62.5 | 333.8 | 928.4 | 62.6 | 324.3 | 978.5 |
Standard Deviation | 3.1 | 15.9 | 58.3 | 1.8 | 6.0 | 39.4 |
Coefficient of Variation (%) | 4.9 | 4.8 | 6.3 | 3.9 | 1.9 | 4.0 |
板内精密度:3 个已知浓度的样本酶标板内重复测定 20 次,评估酶标板内的精密度。
板间精密度:3 个已知浓度的样本酶标板间重复检测 6 次,评估酶标板间的精密度。
Spike Recovery
通过加标 3 个水平的 HIV-1 p24 来评估加标回收。回收率从95%到106%不等,总体平均回收率为101%。
Sample Value
Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
---|---|---|---|---|
Serum |
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
60min
Standard Curve Gradient
7点/3倍
Incubation Count
1
Detectable Sample
Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc.
Dosing Volume
10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Function
人类免疫缺陷病毒 1 (HIV-1) 的 gag 基因编码一种称为 Pr55Gag 的前体蛋白。病毒蛋白酶 PR 切割该前体以产生病毒颗粒组装所需的 p17、p24、p7 和 p6 蛋白。p24 是一种主要的病毒核心结构蛋白。其测量通常用作 HIV-1 感染和病毒载量的指标。
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