NLRP3 Recombinant Rabbit Monoclonal Antibody [PSH19-43] - BSA and Azide free
Overview
Product Name
NLRP3 Recombinant Rabbit Monoclonal Antibody [PSH19-43] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human NLRP3 aa 1-250.
Species Reactivity
Human, Mouse
Validated Applications
WB
Molecular Weight
Predicted band size: 118 kDa
Positive Control
THP-1 cell lysate, THP-1 treated with 100ng/mL LPS for 48 hours cell lysate, RAW264.7 cell lysate, J774A.1 cell lysate, RAW264.7 treated with 10μg/mL LPS for 8 hours cell lysate.
Conjugation
unconjugated
Clone Number
PSH19-43
Product Features
Form
Liquid
Concentration
1 mg/mL.
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:5,000
Target
Function
NLR family pyrin domain containing 3 (NLRP3) (previously known as NACHT, LRR, and PYD domains-containing protein 3 [NALP3] and cryopyrin), is a protein that in humans is encoded by the NLRP3 gene located on the long arm of chromosome 1. NLRP3 is expressed predominantly in macrophages and as a component of the inflammasome, detects products of damaged cells such as extracellular ATP and crystalline uric acid. Activated NLRP3 in turn triggers an immune response. Mutations in the NLRP3 gene are associated with a number of organ specific autoimmune diseases.
Background References
1. Yu T et al. NLRP3 Cys126 palmitoylation by ZDHHC7 promotes inflammasome activation. Cell Rep. 2024 Apr
2. Tengesdal IW et al. NLRP3 and cancer: Pathogenesis and therapeutic opportunities. Pharmacol Ther. 2023 Nov
Subcellular Location
Cytoplasm, Inflammasome, Secreted, Nucleus, Endoplasmic reticulum.
Synonyms
AGTAVPRL antibody
AII/AVP antibody
Angiotensin/vasopressin receptor AII/AVP like antibody
Angiotensin/vasopressin receptor AII/AVP-like antibody
C1orf7 antibody
Caterpiller protein 1.1 antibody
CIAS 1 antibody
CIAS1 antibody
CLR1.1 antibody
Cold autoinflammatory syndrome 1 antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of NLRP3 on different lysates with Rabbit anti-NLRP3 antibody (HA751713) at 1/5,000 dilution.
Lane 1: THP-1 cell lysate
Lane 2: THP-1 treated with 100ng/mL LPS for 48 hours cell lysate
Lane 3: Jurkat cell lysate (low expression)
Lane 4: RAW264.7 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 118 kDa
Observed band size: 118 kDa
Exposure time: 1 minute 50 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751713) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Relative expression (RE)
Western blot analysis of NLRP3 on different lysates with Rabbit anti-NLRP3 antibody (HA751713) at 1/5,000 dilution.
Lane 1: J774A.1 cell lysate
Lane 2: NIH/3T3 cell lysate (low expression)
Lane 3: RAW264.7 cell lysate
Lane 4: RAW264.7 treated with 10μg/mL LPS for 8 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 118 kDa
Observed band size: 118 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751713) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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