Phospho-EGFR (Y992) Recombinant Rabbit Monoclonal Antibody [PSH23-63]

Specification

50μl

100μl

Quota

Contact Us

BIO-STATION LIMITED

Contact: Sanry CHEN

Email: info@bio-station.net

Phone: 852-35650223

Find Local Distributors

Catalog# HA724388

Phospho-EGFR (Y992) Recombinant Rabbit Monoclonal Antibody [PSH23-63]

Application

WB
IF-Cell
FC

Reactivity

Human

BSA and Azide free

HA751904
不含抗保成分

Conjugation

unconjugated

Overview

Product Name

Phospho-EGFR (Y992) Recombinant Rabbit Monoclonal Antibody [PSH23-63]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Tyr992 of human EGFR.

Species Reactivity

Human

Validated Applications

WB, IF-Cell, FC

Molecular Weight

Predicted band size: 134 kDa

Positive Control

A431 treated with 100ng/mL EGF for 30min cell lysate.

Conjugation

unconjugated

Clone Number

PSH23-63

Product Features

Form

Liquid

Concentration

批次浓度查询

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

WB
1:2,000

IF-Cell
1:1,000

FC
1:10,000

Target

Function

The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is a transmembrane protein that is a receptor for members of the epidermal growth factor family (EGF family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). In many cancer types, mutations affecting EGFR expression or activity could result in cancer. Deficient signaling of the EGFR and other receptor tyrosine kinases in humans is associated with diseases such as Alzheimer's, while over-expression is associated with the development of a wide variety of tumors. Interruption of EGFR signalling, either by blocking EGFR binding sites on the extracellular domain of the receptor or by inhibiting intracellular tyrosine kinase activity, can prevent the growth of EGFR-expressing tumours and improve the patient's condition.

Background References

1. Nakamura T et al. LRIG1 inhibits STAT3-dependent inflammation to maintain corneal homeostasis. J Clin Invest 124:385-97 (2014).

2. Furcht CM et al. Multivariate signaling regulation by SHP2 differentially controls proliferation and therapeutic response in glioma cells. J Cell Sci 127:3555-67 (2014).

Subcellular Location

Cell membrane, Nucleus membrane, Nucleus, Endoplasmic reticulum membrane, Golgi apparatus membrane, Endosome.

UNIPROT

SwissProt: P00533 Human

Synonyms

Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody

Cell growth inhibiting protein 40 antibody

Cell proliferation inducing protein 61 antibody

EGF R antibody

EGFR antibody

EGFR_HUMAN antibody

Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody

Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody

Epidermal growth factor receptor antibody

erb-b2 receptor tyrosine kinase 1 antibody

Expand

Images

☑ Cell treatment (CT)

Western blot analysis of Phospho-EGFR (Y992) on different lysates with Rabbit anti-Phospho-EGFR (Y992) antibody (HA724388) at 1/2,000 dilution.

Lane 1: A431 (Human epidermoid carcinoma skin squamous cells) cell lysate
Lane 2: A431 treated with 100ng/mL EGF for 30min cell lysate

Lysates/proteins at 20 µg/Lane.
Exposure time: 12 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724388, 1/2,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 134 kDa
Observed band size: 175 kDa
☑ Cell treatment (CT)

Application: Immunocytochemistry (IF-cell)

Species: Human
Sample: A431 (Human epidermoid carcinoma skin squamous cells) treated with EGF(100ng/ml 30min)

Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.

Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA724388, 1/1,000, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.

Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue).
☑ Cell treatment (CT)

Application: Flow Cytometry (Intra)

Species: Human
Sample: A431 (Human epidermoid carcinoma skin squamous cells) treated with EGF(100ng/ml 30min)

Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature.

Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA724388 (1/10,000) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Phospho-EGFR (Y992) Recombinant Rabbit Monoclonal Antibody [PSH23-63]

Specification

Contact Us

BIO-STATION LIMITED

Overview

Product Name

Antibody Type

Immunogen

Species Reactivity

Validated Applications

Molecular Weight

Positive Control

Conjugation

Clone Number

Product Features

Form

Concentration

Storage Instructions

Storage Buffer

Isotype

Purification Method

Application Dilution

Target

Function

Background References

Subcellular Location

UNIPROT

Synonyms

Images

Products with the same target and pathway

EGFR Recombinant Rabbit Monoclonal Antibody [SP00-86] - BSA and Azide free

Application: WB,IF-Cell,IP,FC

Reactivity: Human,Mouse,Rat,Green monkey

Conjugate: unconjugated