SAA1 + SAA2 Recombinant Rabbit Monoclonal Antibody [PSH21-34] - BSA and Azide free
Catalog# HA751797
SAA1 + SAA2 Recombinant Rabbit Monoclonal Antibody [PSH21-34] - BSA and Azide free
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WB
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IHC-P
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Mouse
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HA724217
含抗保成分
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unconjugated
Overview
Product Name
SAA1 + SAA2 Recombinant Rabbit Monoclonal Antibody [PSH21-34] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within mouse SAA1 aa 1-122.
Product Specificity
This antibody cross-reacts with Mouse SAA1 and SAA2 but not Human SAA1.
Species Reactivity
Mouse
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 14 kDa
Positive Control
Mouse plasma tissue lysate, Mouse serum tissue lysate, mouse liver tissue.
Conjugation
unconjugated
Clone Number
PSH21-34
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IHC-P
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1:200
Target
Function
Serum amyloid A1 (SAA1) is a protein that in humans is encoded by the SAA1 gene. Serum amyloid A protein is a protein that in humans is encoded by the SAA2 gene. SAA1 is a major acute-phase protein mainly produced by hepatocytes in response to infection, tissue injury and malignancy. When released into blood circulation, SAA1 is present as an apolipoprotein associated with high-density lipoprotein (HDL). SAA1 is a major precursor of amyloid A (AA), the deposit of which leads to inflammatory amyloidosis.
Background References
1. Li L et al. Multi-omics data reveal that SAA1 + fibroblasts exacerbate periodontitis by regulating macrophage inflammation and chemotaxis. J Transl Med. 2025 Aug
2. Xie S et al. Small proline-rich protein 2A drives epithelial remodeling in eosinophilic chronic rhinosinusitis with nasal polyps via SAA2 upregulation. J Allergy Clin Immunol. 2025 Oct
Subcellular Location
Secreted.
Synonyms
amyloid A, serum antibody
Amyloid fibril protein AA antibody
Amyloid protein A antibody
MGC111216 antibody
PIG4 antibody
SAA antibody
Serum amyloid A protein antibody
serum amyloid A-1 protein antibody
serum amyloid A1 antibody
TP53I4 antibody
ExpandImages
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Western blot analysis of SAA1 + SAA2 on different lysates with Rabbit anti-SAA1 + SAA2 antibody (HA751797) at 1/5,000 dilution.
Lane 1: Mouse plasma tissue lysate
Lane 2: Mouse serum tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 14 kDa
Observed band size: 12 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751797) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of SAA1 + SAA2 on different lysates with Rabbit anti-SAA1 + SAA2 antibody (HA751797) at 1/5,000 dilution.
Lane 1: no-tagged Mouse SAA1 recombinant protein
Lane 2: Avi-Ltag-His-tagged Mouse SAA2 recombinant protein
Lane 2: Flag-tagged Human SAA1 recombinant protein
Lysates/proteins at 20 ng/Lane.
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751797) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-SAA1 + SAA2 antibody (HA751797) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751797) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"