p38 MAPK Recombinant Rabbit Multiclonal Antibody [PSH03-72]
Catalog# HA722036
p38 MAPK Recombinant Rabbit Multiclonal Antibody [PSH03-72]
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WB
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Human
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Mouse
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Rat
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Monkey
Overview
Product Name
p38 MAPK Recombinant Rabbit Multiclonal Antibody [PSH03-72]
Antibody Type
Recombinant Rabbit multiclonal
Immunogen
Recombinant protein within human MAPK12 aa 1-367 / 367.
Product Specificity
Anti-p38 MAPK Antibody [PSH03-72] detects endogenous levels of total p38α, p38β, p38γ and p38δ MAPK protein.
Species Reactivity
Human, Mouse, Rat, Monkey
Validated Applications
WB
Molecular Weight
Predicted band size: 42 kDa
Positive Control
293T cell lysate, HeLa cell lysate, K-562 cell lysate, COS-1 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate.
Conjugation
unconjugated
Clone Number
PSH03-72
RRID
Product Features
Form
Liquid
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
Applications in Publications
Species in Publications
| Human | See 4 publications below |
| Mouse | See 3 publications below |
Target
Function
A mitogen-activated protein kinase (MAPK or MAP kinase) is a type of protein kinase that is specific to the amino acids serine and threonine (i.e., a serine/threonine-specific protein kinase). MAPKs are involved in directing cellular responses to a diverse array of stimuli, such as mitogens, osmotic stress, heat shock and proinflammatory cytokines. They regulate cell functions including proliferation, gene expression, differentiation, mitosis, cell survival, and apoptosis. MAP kinases are found in eukaryotes only, but they are fairly diverse and encountered in all animals, fungi and plants, and even in an array of unicellular eukaryotes. MAPKs belong to the CMGC (CDK/MAPK/GSK3/CLK) kinase group. The closest relatives of MAPKs are the cyclin-dependent kinases (CDKs). p38 MAP Kinase Antibody detects endogenous levels of total p38 alpha, p38 beta, p38 gamma, p38 delta protein.
Background References
1. Wang J et al. Pan-cancer analysis supports MAPK12 as a potential prognostic and immunotherapeutic target in multiple tumor types, including in THCA. Oncol Lett. 2022 Oct
2. Yang R et al. Prolactin Regulates Ovine Ovarian Granulosa Cell Apoptosis by Affecting the Expression of MAPK12 Gene. Int J Mol Sci. 2023 Jun
Subcellular Location
Cytoplasm, Nucleus, Mitochondrion.
UNIPROT
Synonyms
ERK 6 antibody
ERK-6 antibody
ERK6 antibody
Extracellular signal-regulated kinase 6 antibody
MAP kinase 12 antibody
MAP kinase p38 gamma antibody
MAPK 12 antibody
Mapk12 antibody
Mitogen Activated Protein Kinase 12 antibody
Mitogen activated protein kinase p38 gamma antibody
ExpandImages
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Western blot analysis of p38 MAPK on different lysates with Rabbit anti-p38 MAPK antibody (HA722036) at 1/1,000 dilution.
Lane 1: 293T cell lysate
Lane 2: HeLa cell lysate
Lane 3: K-562 cell lysate
Lane 4: COS-1 cell lysate
Lane 5: Neuro-2a cell lysate
Lane 6: PC-12 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 4 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722036) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
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TGM2 regulated by transcription factor NR3C1 drives p38 MAPK-mediated tumor progression and immune evasion in lung squamous cell carcinoma
Author: Lingge Yang
PMID: 41050683
Journal: Frontiers In Immunology
Application: WB
Reactivity: Human
Publish date: 2025 Sept
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Citation
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Selenomethionine activates MAPK signaling to activate epidermal keratinocyte migration, speeding skin wound healing
Author: Yijing Zhou, Chuanrui Yang, Jieli Bai, Jin Yang, Lin Chen, Qin Yu, Xiaoyan Wen, Yafei Yang
PMID: 40706245
Journal: Biochemical And Biophysical Research Communications
Application: WB
Reactivity: Human
Publish date: 2025 Jul
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Citation
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SH2B1 Promotes Apoptosis in Diabetic Cataract via p38 MAPK Pathway
Author: Xiaohui Jiang, Liming Xu, Boyue Xu, Haotian Peng, Tonghe Yang, Yinying Zhao, Nanxin Wu, Yun-e Zhao
PMID: 39898036
Journal: iScience
Application: WB
Reactivity: Human
Publish date: 2025 Jan
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Citation
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The anti-inflammatory effects of paeonol from Cynanchum paniculatum extracts on LPS-induced macrophage RAW 264.7 cells and Helicobacter pylori-infected gastrointestinal mucosal damaged zebrafish
Author: Mingyun Wang, Jingwen Zhou, Ling Li, Tong Zhang, Yiqiong Pu
PMID: 39987996
Journal: Journal Of Ethnopharmacology
Application: WB
Reactivity: Mouse
Publish date: 2025 Feb
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Citation
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Rv2741 Promotes Mycobacterium Survival by Modulating Macrophage Function via the IL-1α-MAPK Axis
Author: Xintong He, Yonglin He, Xichuan Deng, Nan Lu, Anlong Li, Sijia Gao, Shiyan He, Yuran Wang, Nanzhe Fu, Zijie Wang, Yuxin Nie, Lei Xu
PMID: 40009799
Journal: ACS Infectious Diseases
Application: WB
Reactivity: Mouse
Publish date: 2025 Feb
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Citation
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Jiawei Taohe Chengqi Decoction attenuates CCl4 induced hepatic fibrosis by inhibiting HSCs activation via TGF-β1/CUGBP1 and IFN-γ/Smad7 pathway
Author: Linmao Ye, Jiaxin Huang, Xiaofan Liang, Wenqin Guo, Xiguang Sun, Chang Shao, Yi He, Junjie Zhang
PMID: 39094440
Journal: Phytomedicine
Application: WB
Reactivity: Mouse,Human
Publish date: 2024 Jul
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Citation
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