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Myc tag Mouse Monoclonal Antibody [A3-B4]

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Catalog# EM31105

Myc tag Mouse Monoclonal Antibody [A3-B4]

Application

  • WB

  • IF-Cell

  • ELISA

  • FC

  • IP

Reactivity

  • Species independent

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Myc tag Mouse Monoclonal Antibody [A3-B4]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Synthetic peptide immune sequence is EQKLISEEDLC-KLH.

Species Reactivity

Species independent

Validated Applications

WB, IF-Cell, ELISA, FC, IP

Positive Control

Myc-tagged recombinant protein.

Conjugation

unconjugated

Clone Number

A3-B4

RRID

AB_3068994

Product Features

Form

Liquid

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG2b

Purification Method

Protein G affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IF-Cell

  • 1:50

  • FC

  • 1:50-1:100

  • IP

  • 2-5 µg/ml.

Target

Function

Myc gene encodes for a transcription factor that is believed to regulate expression of 15% of all genes through binding on Enhancer Box sequences (E-boxes) and recruiting histone acetyltransferases (HATs). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. This Myc-Tag antibody detects Myc-tagged fusion proteins.

Background References

1. "A quantitative atlas of mitotic phosphorylation."Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008)

2. "Transactivation of gene expression by Myc is inhibited by mutation at the phosphorylation sites Thr-58 and Ser-62."Gupta S., Seth A., Davis R.J. Proc. Natl. Acad. Sci. U.S.A. 90:3216-3220(1993)

Synonyms

avian myelocytomatosis viral oncogene homolog antibody

bHLHe39 antibody

c-Myc antibody

class E basic helix-loop-helix protein 39 antibody

MRTL antibody

MYC antibody

Myc Epitope Tag antibody

myc proto-oncogene protein antibody

myc-related translation/localization regulatory factor antibody

oncogene c-Myc antibody

Expand

Images

  • Western blot analysis of Myc tag on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/EM31105" style="font-weight: bold;text-decoration: underline;">EM31105</a>, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:5,000 dilution was used for 1 hour at room temperature.<br />Positive control: <br />Lane 1: C-terminal Myc-tagged recombinant protein<br />Lane 2: N-terminal Myc-tagged recombinant protein

    Western blot analysis of Myc tag on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM31105, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: C-terminal Myc-tagged recombinant protein
    Lane 2: N-terminal Myc-tagged recombinant protein

  • ICC staining of Myc tag in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/EM31105" style="font-weight: bold;text-decoration: underline;">EM31105</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of Myc tag in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM31105, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  • Myc tag was immunoprecipitated in 2µg C terminal Myc Tag fusion protein lysate with <a href="/products/EM31105" style="font-weight: bold;text-decoration: underline;">EM31105</a> at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using <a href="/products/R1208-1" style="font-weight: bold;text-decoration: underline;">R1208-1</a> at 1/1,000 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 60 mins at room temperature.<br /><br />Lane 1: Myc Tag fusion protein lysate (input).<br />Lane 2: <a href="/products/EM1902-38" style="font-weight: bold;text-decoration: underline;">EM1902-38</a> IP in Myc Tag fusion protein lysate.<br />Lane 3: <a href="/products/EM31105" style="font-weight: bold;text-decoration: underline;">EM31105</a> IP in Myc Tag fusion protein lysate.<br />Lane 4: Mouse IgG instead of <a href="/products/EM31105" style="font-weight: bold;text-decoration: underline;">EM31105</a> in Myc Tag fusion protein lysate.<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST

    Myc tag was immunoprecipitated in 2µg C terminal Myc Tag fusion protein lysate with EM31105 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using R1208-1 at 1/1,000 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 60 mins at room temperature.

    Lane 1: Myc Tag fusion protein lysate (input).
    Lane 2: EM1902-38 IP in Myc Tag fusion protein lysate.
    Lane 3: EM31105 IP in Myc Tag fusion protein lysate.
    Lane 4: Mouse IgG instead of EM31105 in Myc Tag fusion protein lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST

  • Myc tag was immunoprecipitated in 2µg N terminal Myc Tag fusion protein lysate with <a href="/products/EM31105" style="font-weight: bold;text-decoration: underline;">EM31105</a> at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using <a href="/products/R1208-1" style="font-weight: bold;text-decoration: underline;">R1208-1</a> at 1/1,000 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 60 mins at room temperature.<br /><br />Lane 1: Myc Tag fusion protein lysate (input).<br />Lane 2: <a href="/products/EM1902-38" style="font-weight: bold;text-decoration: underline;">EM1902-38</a> IP in Myc Tag fusion protein lysate.<br />Lane 3: <a href="/products/EM31105" style="font-weight: bold;text-decoration: underline;">EM31105</a> IP in Myc Tag fusion protein lysate.<br />Lane 4: Mouse IgG instead of <a href="/products/EM31105" style="font-weight: bold;text-decoration: underline;">EM31105</a> in Myc Tag fusion protein lysate.<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST

    Myc tag was immunoprecipitated in 2µg N terminal Myc Tag fusion protein lysate with EM31105 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using R1208-1 at 1/1,000 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 60 mins at room temperature.

    Lane 1: Myc Tag fusion protein lysate (input).
    Lane 2: EM1902-38 IP in Myc Tag fusion protein lysate.
    Lane 3: EM31105 IP in Myc Tag fusion protein lysate.
    Lane 4: Mouse IgG instead of EM31105 in Myc Tag fusion protein lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Filter:
  1. WB
  2. IF
  3. IP
  4. Co-IP
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