IP3 Receptor Recombinant Rabbit Monoclonal Antibody [JA11-35]
Catalog# ET1704-77
IP3 Receptor Recombinant Rabbit Monoclonal Antibody [JA11-35]
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WB
-
IHC-P
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IHC-Fr
-
IF-Tissue
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Human
-
Mouse
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Rat
Overview
Product Name
IP3 Receptor Recombinant Rabbit Monoclonal Antibody [JA11-35]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human IP3 Receptor aa 2190-2280 / 2758.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IHC-Fr, IF-Tissue
Molecular Weight
Predicted band size: 314 kDa
Positive Control
Rat brain tissue lysate, Mouse brain tissue lysate, human cerebellum tissue, mouse cerebellum tissue, rat cerebellum tissue.
Conjugation
unconjugated
Clone Number
JA11-35
RRID
Product Features
Form
Liquid
Concentration
1ug/ul
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000-1:2,000
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IHC-P
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1:1,000
-
IHC-Fr
-
1:500
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IF-Tissue
-
1:500
Target
Function
Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5-trisphosphate. Involved in the regulation of epithelial secretion of electrolytes and fluid through the interaction with AHCYL1. Plays a role in ER stress-induced apoptosis. Cytoplasmic calcium released from the ER triggers apoptosis by the activation of CaM kinase II, eventually leading to the activation of downstream apoptosis pathways.
Background References
1. Cao X. et. al. ORP4L couples IP(3) to ITPR1 in control of endoplasmic reticulum calcium release. FASEB J. 2019 Dec
2. Synofzik M. et. al. De novo ITPR1 variants are a recurrent cause of early-onset ataxia, acting via loss of channel function. Eur J Hum Genet. 2018 Nov
Sequence Similarity
Belongs to the InsP3 receptor family.
Tissue Specificity
Widely expressed.
Post-translational Modification
Phosphorylated on tyrosine residues.; Ubiquitination at multiple lysines targets ITPR1 for proteasomal degradation. Approximately 40% of the ITPR1-associated ubiquitin is monoubiquitin, and polyubiquitins are both 'Lys-48'- and 'Lys-63'-linked (By similarity).; Phosphorylated by cAMP kinase (PKA). Phosphorylation prevents the ligand-induced opening of the calcium channels. Phosphorylation by PKA increases the interaction with inositol 1,4,5-trisphosphate and decreases the interaction with AHCYL1.; Palmitoylated by ZDHHC6 in immune cells, leading to regulate ITPR1 stability and function.
Subcellular Location
Endoplasmic reticulum membrane, secretory vesicle membrane, perinuclear region.
Synonyms
4 antibody
5-trisphosphate receptor antibody
5-trisphosphate receptor type 1 antibody
DKFZp313E1334 antibody
DKFZp313N1434 antibody
inositol 1 4 5 triphosphate receptor type 1 antibody
Inositol 1 4 5 trisphosphate Receptor Type 1 antibody
Inositol 1 antibody
InsP3R1 antibody
IP3 antibody
ExpandImages
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Western blot analysis of IP3 Receptor on different lysates with Rabbit anti-IP3 Receptor antibody (ET1704-77) at 1/1,000 dilution.
Lane 1: Rat brain tissue lysate
Lane 2: Mouse brain tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 314 kDa
Observed band size: 314 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-77) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-IP3 Receptor antibody (ET1704-77) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-77) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-IP3 Receptor antibody (ET1704-77) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-77) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-IP3 Receptor antibody (ET1704-77) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-77) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Application: IHC-Fr
Species: Mouse
Site: Cerebellum
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. -
Application: IHC-Fr
Species: Rat
Site: cerebellum
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
Application: IF-tissue
Species: Mouse
Site: Cerebellum
Sample: Paraffin-embedded section
Antibody concentration: 1/500 -
Application: IF-Tissue
Species: Rat
Site: cerebellum
Sample: Paraffin-embedded section
Antibody concentration: 1/500
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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