VGLUT1 Recombinant Antibody [PSH10-53] - Guinea pig IgG2 (Chimeric)
Overview
Product Name
VGLUT1 Recombinant Antibody [PSH10-53] - Guinea pig IgG2 (Chimeric)
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Recombinant protein within Mouse VGLUT1 aa 1-560.
Species Reactivity
Mouse, Rat
Validated Applications
IHC-Fr, WB, IF-Tissue
Molecular Weight
Predicted band size: 62 kDa
Positive Control
Mouse brain tissue lysate.
Conjugation
unconjugated
Clone Number
PSH10-53
Product Features
Form
Liquid
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Purification Method
Protein A affinity purified.
Application Dilution
-
IHC-Fr
-
1:500
-
WB
-
1:5,000
-
IF-Tissue
-
1:500
Target
Function
Vesicular glutamate transporter 1 (VGLUT1) is a protein that in humans is encoded by the SLC17A7 gene. The protein encoded by this gene is a vesicle-bound, sodium-dependent phosphate transporter that is specifically expressed in the neuron-rich regions of the brain. It is preferentially associated with the membranes of synaptic vesicles and functions in glutamate transport. The protein shares 82% identity with the differentiation-associated Na-dependent inorganic phosphate cotransporter and they appear to form a distinct class within the Na+/Pi cotransporter family.
Background References
1. Souter EA et al. Disruption of VGLUT1 in Cholinergic Medial Habenula Projections Increases Nicotine Self-Administration. eNeuro. 2022 Jan
2. Jin S et al. Molecular Profiling of VGluT1 AND VGluT2 Ventral Subiculum to Nucleus Accumbens Shell Projections. Neurochem Res. 2023 Aug
Subcellular Location
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Cell membrane, Synapse, synaptosome.
Synonyms
BNPI antibody
Brain specific Na (+) dependent inorganic phosphate cotransporter antibody
Brain specific Na dependent inorganic phosphate cotransporter antibody
Brain-specific Na(+)-dependent inorganic phosphate cotransporter antibody
Slc17a7 antibody
Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 antibody
Solute carrier family 17 member 7 antibody
Vesicular glutamate transporter 1 antibody
VGLU1_HUMAN antibody
VGLUT 1 antibody
ExpandImages
-
-
-
Western blot analysis of VGLUT1 on different lysates with Guinea pig anti-VGLUT1 antibody (HA601449) at 1/5,000 dilution.
Lane 1: Mouse brain tissue lysate (no heat) (20 µg/Lane)
Lane 2: Mouse lung tissue lysate (no heat) (negative) (20 µg/Lane)
Notice: no heat means the lysate is not boiled.
Predicted band size: 62 kDa
Observed band size: 62 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601449) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021) at 1/5,000 dilution was used for 1 hour at room temperature. -
Application: Immunofluorescence (IF-tissue)
Species: Mouse
Tissue: Hippocampus
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.
Wash buffer: 1× PBST
Endogenous peroxidase blocking: 3% H2O2, 10 minutes.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: VGLUT1, 1/500 (HA601449, Guinea pig, Red), GAD67, 1:500 (HA601370, Mouse, Green), overnight at 4℃.
Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), Goat anti-Guinea pig IgG (iFluor™ 594, HA1143), 1.5 hours at room temperature. -
Application: Immunofluorescence (IF-tissue)
Species: Rat
Tissue: Hippocampus
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.
Wash buffer: 1× PBST
Endogenous peroxidase blocking: 3% H2O2, 10 minutes.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: VGLUT1, 1/500 (HA601449, Guinea pig, Red), GAD67, 1:500 (HA601370, Mouse, Green), overnight at 4℃.
Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), Goat anti-Guinea pig IgG (iFluor™ 594, HA1143), 1.5 hours at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"