gamma Adaptin Recombinant Rabbit Monoclonal Antibody [PSH02-65]
Overview
Product Name
gamma Adaptin Recombinant Rabbit Monoclonal Antibody [PSH02-65]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human gamma Adaptin aa 623-822 / 822.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 91 kDa
Positive Control
HeLa cell lysate, 293T cell lysate, Jurkat cell lysate, HepG2 cell lysate, Raji cell lysate, MCF7 cell lysate, A549 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, mouse kidney tissue lysate, mouse liver tissue lysate, rat kidney tissue lysate, rat liver tissue lysate, mouse kidney tissue, rat brain tissue.
Conjugation
unconjugated
Clone Number
PSH02-65
RRID
Product Features
Form
Liquid
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
-
IHC-P
-
1:200
Target
Function
Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. In association with AFTPH/aftiphilin in the aftiphilin/p200/gamma-synergin complex, involved in the trafficking of transferrin from early to recycling endosomes, and the membrane trafficking of furin and the lysosomal enzyme cathepsin D between the trans-Golgi network (TGN) and endosomes.
Background References
1. Usmani MA, Ahmed ZM, Magini P, et al. De novo and bi-allelic variants in AP1G1 cause neurodevelopmental disorder with developmental delay, intellectual disability, and epilepsy. Am J Hum Genet. 2021 Jul 1;108(7):1330-1341.
2. Tao X, Lu Y, Qiu S, et al. AP1G1 is involved in cetuximab-mediated downregulation of ASCT2-EGFR complex and sensitization of human head and neck squamous cell carcinoma cells to ROS-induced apoptosis. Cancer Lett. 2017 Nov 1; 408: 33-42.
Subcellular Location
Golgi apparatus, Cytoplasmic vesicle, clathrin-coated vesicle membrane, Cytoplasm, perinuclear region,
Synonyms
Adapter-related protein complex 1 subunit gamma-1 antibody
Adaptor protein complex AP 1 gamma 1 subunit antibody
Adaptor protein complex AP 1 subunit gamma 1 antibody
Adaptor protein complex AP-1 subunit gamma-1 antibody
Adaptor related protein complex 1 gamma 1 subunit antibody
ADTG antibody
AP 1 complex subunit gamma 1 antibody
AP-1 complex subunit gamma-1 antibody
AP1G1 antibody
AP1G1_HUMAN antibody
ExpandImages
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Western blot analysis of gamma Adaptin on different lysates with Rabbit anti-gamma Adaptin antibody (HA721843) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: 293T cell lysate (20 µg/Lane)
Lane 3: Jurkat cell lysate (20 µg/Lane)
Lane 4: HepG2 cell lysate (20 µg/Lane)
Lane 5: Raji cell lysate (20 µg/Lane)
Lane 6: MCF7 cell lysate (20 µg/Lane)
Lane 7: A549 cell lysate (20 µg/Lane)
Lane 8: NIH/3T3 cell lysate (20 µg/Lane)
Lane 9: RAW264.7 cell lysate (20 µg/Lane)
Lane 10: PC-12 cell lysate (20 µg/Lane)
Lane 11: Mouse kidney tissue lysate (40 µg/Lane)
Lane 12: Mouse liver tissue lysate (40 µg/Lane)
Lane 13: Rat kidney tissue lysate (40 µg/Lane)
Lane 14: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 91 kDa
Observed band size: 91 kDa
Exposure time: 1 minute 46 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721843) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-gamma Adaptin antibody (HA721843) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721843) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-gamma Adaptin antibody (HA721843) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721843) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Knockdown (KD)
Western blot analysis of gamma Adaptin on different lysates with Rabbit anti-gamma Adaptin antibody (HA721843) at 1/1,000 dilution.
Lane 1: HEK-293-si NT cell lysate
Lane 2: HEK-293-si gamma Adaptin cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 91 kDa
Observed band size: 91 kDa
Exposure time: 2 minutes 18 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721843) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"