Somatostatin Receptor 2 Recombinant Rabbit Monoclonal Antibody [JE03-37]
Catalog# HA722521
Somatostatin Receptor 2 Recombinant Rabbit Monoclonal Antibody [JE03-37]
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WB
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IHC-P
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IF-Tissue
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IHC-Fr
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Human
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Mouse
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Rat
Overview
Product Name
Somatostatin Receptor 2 Recombinant Rabbit Monoclonal Antibody [JE03-37]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human Somatostatin Receptor 2 aa 320-369 / 369.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IF-Tissue, IHC-Fr
Molecular Weight
Predicted band size: 41 kDa
Positive Control
SH-SY5Y cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, mouse brain tissue, human brain tissue, rat brain tissue.
Conjugation
unconjugated
Clone Number
JE03-37
Product Features
Form
Liquid
Concentration
1ug/ul
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:200
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IF-Tissue
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1:100
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IHC-Fr
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1:500
Target
Function
Receptor for somatostatin-14 and -28. This receptor is coupled via pertussis toxin sensitive G proteins to inhibition of adenylyl cyclase. In addition it stimulates phosphotyrosine phosphatase and PLC via pertussis toxin insensitive as well as sensitive G proteins. Inhibits calcium entry by suppressing voltage-dependent calcium channels. Acts as the functionally dominant somatostatin receptor in pancreatic alpha- and beta-cells where it mediates the inhibitory effect of somatostatin-14 on hormone secretion. Inhibits cell growth through enhancement of MAPK1 and MAPK2 phosphorylation and subsequent up-regulation of CDKN1B. Stimulates neuronal migration and axon outgrowth and may participate in neuron development and maturation during brain development. Mediates negative regulation of insulin receptor signaling through PTPN6. Inactivates SSTR3 receptor function following heterodimerization.
Background References
1. Kailey B., van de Bunt M., Cheley S., Johnson P.R., MacDonald P.E., Gloyn A.L., Rorsman P., Braun M. SSTR2 is the functionally dominant somatostatin receptor in human pancreatic beta- and alpha-cells. Am. J. Physiol. 303:E1107-E1116 (2012)
2. Parry J.J., Chen R., Andrews R., Lears K.A., Rogers B.E. Identification of critical residues involved in ligand binding and G protein signaling in human somatostatin receptor subtype 2. Endocrinology 153:2747-2755 (2012)
Subcellular Location
Cell membrane.
Synonyms
Somatostatin receptor type 2 antibody
SRIF-1 antibody
SRIF1 antibody
SS-2-R antibody
SS2-R antibody
SS2R antibody
SSR2_HUMAN antibody
SST2 antibody
SSTR2 antibody
Images
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Immunofluorescence analysis of frozen mouse brain tissue with Rabbit anti-Somatostatin Receptor 2 antibody (HA722521) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722521, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Immunofluorescence analysis of frozen mouse brain tissue with Rabbit anti-Somatostatin Receptor 2 antibody (HA722521) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722521, red) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Somatostatin Receptor 2 antibody (HA722521) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722521) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Somatostatin Receptor 2 antibody (HA722521) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722521) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Somatostatin Receptor 2 antibody (HA722521) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722521) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunofluorescence analysis of paraffin-embedded rat brain tissue labeling Somatostatin Receptor 2 with Rabbit anti-Somatostatin Receptor 2 antibody (HA722521) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722521, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Western blot analysis of Somatostatin Receptor 2 on different lysates with Rabbit anti-Somatostatin Receptor 2 antibody (HA722521) at 1/1,000 dilution.
Lane 1: SH-SY5Y cell lysate
Lane 2: SH-SY5Y cell lysate (no heat)
Lane 3: Mouse brain tissue lysate (no heat)
Lane 4: Rat brain tissue lysate (no heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 41 kDa
Observed band size: 60-75 kDa
Exposure time: Lane 1-2: 2 minutes; Lane 3-4: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722521) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"