MHC Class II Recombinant Rabbit Monoclonal Antibody [JA10-94] - BSA and Azide free
Catalog# HA750404
MHC Class II Recombinant Rabbit Monoclonal Antibody [JA10-94] - BSA and Azide free
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WB
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IP
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IF-Cell
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IF-Tissue
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IHC-P
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Human
Overview
Product Name
MHC Class II Recombinant Rabbit Monoclonal Antibody [JA10-94] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human HLA-DPB1 aa 11-60 / 258.
Species Reactivity
Human
Validated Applications
WB, IP, IF-Cell, IF-Tissue, IHC-P
Molecular Weight
Predicted band size: 29 kDa
Positive Control
Daudi cell lysates, human tonsil tissue.
Conjugation
unconjugated
Clone Number
JA10-94
Product Features
Form
Liquid
Concentration
1ug/ul
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IF-Cell
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1:50-1:100
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IF-Tissue
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1:50-1:100
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IHC-P
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1:1,000
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IP
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Use at an assay dependent concentration.
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mIHC
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1:2,000
Target
Function
Major histocompatibility complex (MHC) molecules, also designated human leukocyte antigen (HLA) molecules, are cell-surface receptors that bind foreign peptides and present them to T lymphocytes. MHC class I molecules consist of two polypeptide chains, an α or heavy chain and β-2-Microglobulin, a non-covalently associated protein. Cytotoxic T lymphocytes bind antigenic peptides presented by MHC class I molecules. Antigens that bind to MHC class I molecules are typically eight to ten residues in length and are stabilized in a peptide binding groove. MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an α and β chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumu-lating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes.
Background References
1. Feng M et al. ALV-J strain SCAU-HN06 induces innate immune responses in chicken primary monocyte-derived macrophages. Poult Sci N/A:N/A (2016).
2. Arebro J et al. Antigen-presenting epithelial cells can play a pivotal role in airway allergy. J Allergy Clin Immunol N/A:N/A (2015).
Sequence Similarity
Belongs to the MHC class II family.
Subcellular Location
Cell membrane. Endoplasmic reticulum membrane.
UNIPROT
Synonyms
D6S221E antibody
DMA antibody
DMB antibody
DP beta 1 antibody
DP beta 1 chain antibody
DP(W4) beta chain antibody
DPB 1 antibody
DPB1 antibody
DPB1_HUMAN antibody
DRB antibody
ExpandImages
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Western blot analysis of MHC Class II on Daudi cell lysates with Rabbit anti-MHC Class II antibody (HA750404) at 1/500 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 1 minute;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750404) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-MHC Class II antibody (HA750404) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750404) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Fluorescence multiplex immunohistochemical analysis of human tonsil (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-HLA-DBP1 (HA750404, Green) and anti-Tryptase (ET1610-64, Red) on tonsil. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in two rounds of staining: in the order of ET1704-13 (1/2,000 dilution) and ET1610-64 (1/5,000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Zeiss Observer 7 Inverted Fluorescence Microscope.
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Fluorescence multiplex immunohistochemical analysis of Human tonsil (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-BCL6 (HA601083, Red), anti-HLA-DPB1 (HA750404, Green), anti-Tryptase (ET1610-64, White), anti-CD20 (HA721138, Magenta) and anti-CD45 (ET7111-03, Yellow) on tonsil. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in five rounds of staining: in the order of HA601083 (1/200 dilution), ET1704-13 (1/2,000 dilution), ET1610-64 (1/5,000 dilution), HA721138 (1/2,000 dilution) and ET7111-03 (1/500 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
MHC Class II Recombinant Rabbit Monoclonal Antibody [JA10-94]
Application: WB,IP,IF-Cell,IF-Tissue,IHC-P,mIHC
Reactivity: Human
Conjugate: unconjugated
