Tau Recombinant Rabbit Monoclonal Antibody [PSH05-50] - BSA and Azide free
Catalog# HA751009
Tau Recombinant Rabbit Monoclonal Antibody [PSH05-50] - BSA and Azide free
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WB
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IHC-P
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IF-Tissue
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IP
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Mouse
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Rat
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Pig
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HA722288
含抗保成分
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unconjugated
Overview
Product Name
Tau Recombinant Rabbit Monoclonal Antibody [PSH05-50] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human Tau aa 571-620 / 758.
Species Reactivity
Mouse, Rat, Pig
Validated Applications
WB, IHC-P, IF-Tissue, IP
Target Molecular Weight
Predicted band size: 79 kDa
Positive Control
Mouse brain tissue lysate, Mouse cerebellum tissue lysate, Rat brain tissue lysate, Rat cerebellum tissue lysate, Pig brain tissue lysate, rat kidney tissue, rat colon tissue.
Conjugation
unconjugated
Clone Number
PSH05-50
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
The tau proteins (abbreviated from tubulin associated unit are a group of six highly soluble protein isoforms produced by alternative splicing from the gene MAPT (microtubule-associated protein tau). They have roles primarily in maintaining the stability of microtubules in axons and are abundant in the neurons of the central nervous system (CNS), where the cerebral cortex has the highest abundance. They are less common elsewhere but are also expressed at very low levels in CNS astrocytes and oligodendrocytes. Pathologies and dementias of the nervous system such as Alzheimer's disease and Parkinson's disease are associated with tau proteins that have become hyperphosphorylated insoluble aggregates called neurofibrillary tangles. The tau proteins were identified in 1975 as heat-stable proteins essential for microtubule assembly, and since then they have been characterized as intrinsically disordered proteins.
Background References
1. Karikari TK et al. Blood phospho-tau in Alzheimer disease: analysis, interpretation, and clinical utility. Nat Rev Neurol. 2022 Jul
2. Palmqvist S et al. Prediction of future Alzheimer\'s disease dementia using plasma phospho-tau combined with other accessible measures. Nat Med. 2021 Jun
Subcellular Location
Cytoplasm, cytosol, Cell membrane, cytoskeleton, Cell projection, axon, dendrite, Secreted.
Synonyms
AI413597 antibody
AW045860 antibody
DDPAC antibody
FLJ31424 antibody
FTDP 17 antibody
G protein beta1/gamma2 subunit interacting factor 1 antibody
MAPT antibody
MAPTL antibody
MGC134287 antibody
MGC138549 antibody
ExpandImages
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☑ Relative expression (RE)
This data was developed using HA722288, the same antibody clone in a different buffer formulation.
Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (HA722288) at 1/5,000 dilution.
Lane 1: Mouse brain tissue lysate (20 µg/Lane)
Lane 2: Mouse spleen tissue lysate (negative) (20 µg/Lane)
Lane 3: Mouse cerebellum tissue lysate (20 µg/Lane)
Lane 4: Mouse testis tissue lysate (negative) (20 µg/Lane)
Lane 5: Rat brain tissue lysate (20 µg/Lane)
Lane 6: Rat spleen tissue lysate (negative) (20 µg/Lane)
Lane 7: Rat cerebellum tissue lysate (20 µg/Lane)
Lane 8: Rat testis tissue lysate (negative) (20 µg/Lane)
Lane 9: Pig brain tissue lysate (20 µg/Lane)
Lane 10: Pig spleen tissue lysate (negative) (20 µg/Lane)
Observed band size: 50-60 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722288) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Negative control : Spleen, Testis (PMID: 24309898, PMID: 24386422) -
This data was developed using HA722288, the same antibody clone in a different buffer formulation.
Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (HA722288) at 1/1,000 dilution.
Lane 1: Mouse brain tissue lysate (hot lysis)
Lane 2: Rat brain tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 79 kDa
Observed band size: 50-70 kDa
Exposure time: 1 minute 50 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722288) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Relative expression (RE)
This data was developed using HA722288, the same antibody clone in a different buffer formulation.
Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (HA722288) at 1/5,000 dilution.
Lane 1: Mouse brain tissue lysate (40 µg/Lane)
Lane 2: HeLa cell lysate (negative) (20 µg/Lane)
Lane 3: Rat brain tissue lysate (no heat) (40 µg/Lane)
Notice: no heat means the lysate is not boiled.
Predicted band size: 79 kDa
Observed band size: 50-70 kDa
Exposure time: 14 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722288) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA722288, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Tau antibody (HA722288) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722288) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA722288, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-Tau antibody (HA722288) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722288) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA722288, the same antibody clone in a different buffer formulation.
Tau was immunoprecipitated from 0.2 mg mouse brain tissue lysate with HA722288 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722288 at 1/5,000 dilution. Anti-Rabbit IgG for IP, AlpSdAbs® VHH(HRP) (025-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: Mouse brain tissue lysate (input)
Lane 2: HA722288 IP in mouse brain tissue lysate
Lane 3: Rabbit IgG instead of HA722288 in mouse brain tissue lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 50 seconds; ECL: K1802
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"