Inhibin alpha Recombinant Rabbit Monoclonal Antibody [PD01-29] - BSA and Azide free
Catalog# HA751016
Inhibin alpha Recombinant Rabbit Monoclonal Antibody [PD01-29] - BSA and Azide free
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IHC-P
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WB
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Human
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Mouse
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Rat
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HA722301
含抗保成分
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unconjugated
Overview
Product Name
Inhibin alpha Recombinant Rabbit Monoclonal Antibody [PD01-29] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Inhibin alpha aa 233-366.
Species Reactivity
Human, Mouse, Rat
Validated Applications
IHC-P, WB
Molecular Weight
Predicted band size: 40 kDa
Positive Control
Mouse ovary tissue lysate, mouse testis tissue lysate, rat ovary tissue lysate, rat testis tissue lysate, human adrenal gland tissue, human ovary granular cell tumor tissue.
Conjugation
unconjugated
Clone Number
PD01-29
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-P
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1:500-1:1,000
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WB
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1:1,000
Target
Function
Inhibin, alpha, also known as INHA, is a protein which in humans is encoded by the INHA gene. The inhibin alpha subunit joins either the beta A or beta B subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumour-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. However, in prostate cancer, expression of the inhibin alpha-subunit gene was suppressed and was not detectable in poorly differentiated tumor cells. Furthermore, because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone.
Background References
1. Mete O et al. Significance of Alpha-inhibin Expression in Pheochromocytomas and Paragangliomas. Am J Surg Pathol. 2021 Sep
2. Huang L et al. Elevated expression of inhibin alpha gene in sterile allotriploid crucian carp. Gen Comp Endocrinol. 2021 Oct
Subcellular Location
Secreted.
Synonyms
A inhibin subunit antibody
A inhibin subunit precursor antibody
AW55578 antibody
IHA antibody
inhA antibody
INHA_HUMAN antibody
Inhibin alpha chain antibody
Inhibin alpha chain precursor antibody
Inhibin alpha subunit antibody
Images
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Western blot analysis of Inhibin alpha on different lysates with Rabbit anti-Inhibin alpha antibody (HA751016) at 1/1,000 dilution.
Lane 1: Mouse ovary tissue lysate
Lane 2: Mouse testis tissue lysate
Lane 3: Rat ovary tissue lysate
Lane 4: Rat testis tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 40 kDa
Observed band size: 40-45 kDa
Exposure time: 16 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751016) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue with Rabbit anti-Inhibin alpha antibody (HA751016) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751016) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human ovary granular cell tumor tissue with Rabbit anti-Inhibin alpha antibody (HA751016) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751016) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"