FCER1G Recombinant Rabbit Monoclonal Antibody [PSH24-96] - BSA and Azide free
Catalog# HA751962
FCER1G Recombinant Rabbit Monoclonal Antibody [PSH24-96] - BSA and Azide free
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WB
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IF-Cell
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IHC-P
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FC
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IP
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Human
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Mouse
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Rat
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HA724491
含抗保成分
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unconjugated
Overview
Product Name
FCER1G Recombinant Rabbit Monoclonal Antibody [PSH24-96] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human FCER1G aa 31-86.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P, FC, IP
Molecular Weight
Predicted band size: 10 kDa
Positive Control
THP-1 (Human acute monoblastic leukemia cell) cell lysate, RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate.
Conjugation
unconjugated
Clone Number
PSH24-96
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IF-Cell
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1:500
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IHC-P
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1:200-1:1,000
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FC
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1:1,000
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IP
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1-2μg/sample
Target
Function
Fc fragment of IgE, high affinity I, receptor for; gamma polypeptide is gamma chain of high affinity IgE receptor. This protein is encoded by the FCER1G gene in humans. The high affinity IgE receptor, FcεRI, is a key molecule involved in allergic reactions. It is a tetramer composed of 1 alpha, 1 beta, and 2 gamma chains. The gamma chains are also subunits of other Fc receptors.
Background References
1. Podgórska D et al. FCER1G Gene Hypomethylation in Patients with Rheumatoid Arthritis. J Clin Med. 2022 Aug
2. Hu Y et al. FCER1G as a novel immune-associated blood biomarker in cardiogenic stroke. Heliyon. 2024 Jun
Subcellular Location
Cell membrane.
Synonyms
High affinity immunoglobulin epsilon receptor subunit gamma
Fc receptor gamma-chain (FcRgamma)
Fc-epsilon RI-gamma
IgE Fc receptor subunit gamma (FceRI gamma)
FCER1G
Images
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☑ Relative expression (RE)
Western blot analysis of FCER1G on different lysates with Rabbit anti-FCER1G antibody (HA751962) at 1/5,000 dilution.
Lane 1: THP-1 (Human acute monoblastic leukemia cell) cell lysate
Lane 2: 293T (Human embryonic kidney cell) cell lysate
Lane 3: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate
Lane 4: Neuro-2a (Mouse brain neuroblastoma cell) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 7 seconds; ECL: K1801
Negative expression of FCER1G protein in 293T and Neuro-2a cells is consistent with the predicted expression pattern.
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751962, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 10 kDa
Observed band size: 12 kDa -
Application: Immunocytochemistry (IF-cell)
Species: Human
Sample: THP-1 (Human acute monoblastic leukemia cell)/293T (Human embryonic kidney cell)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA751962, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).
Negative expression of FCER1G protein in 293T cell is consistent with the predicted expression pattern -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Ovarian serous carcinoma
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA751962, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Prostate carcinoma
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA751962, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Thymus
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA751962, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Spleen
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA751962, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Thymus
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA751962, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Spleen
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA751962, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Thymus
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA751962, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Spleen
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA751962, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
☑ Relative expression (RE)
Application: Flow Cytometry (Intra)
Species: Human
Sample: THP-1 (Human acute monoblastic leukemia cell)/293T (Human embryonic kidney cell)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA751962(1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.
Negative expression of FCER1G protein in 293T cell is consistent with the predicted expression pattern -
Immunoprecipitation (IP)
FCER1G was immunoprecipitated in 0.2 mg THP-1 (Human acute monoblastic leukemia cell) cell lysate with HA751962 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751962 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: THP-1 cell lysate (input)
Lane 2: HA751962 IP in THP-1 cell lysate
Lane 3: Rabbit IgG instead of HA751962 in THP-1 cell lysate
Exposure time: 46 seconds
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary dilution: HA751962, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature
Predicted band size: 10 kDa
Observed band size: 12 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"