Human Livin/BIRC7, C-His Tag Protein

Specification

20μg

50μg

100μg

Quota

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Catalog# HA211325

Human Livin/BIRC7, C-His Tag Protein

Product Information

Product name

Human Livin/BIRC7, C-His Tag Protein

Catalog No.

HA211325

Predicted molecular mass

35.2 kD

Protein construction description

A DNA sequence encoding the human Livin/BIRC7 protein (Q96CA5-1) (Met 1-Ser 298) was expressed with a His tag at the C-terminus.

Accession

Q96CA5-1

Protein construction

Livin/BIRC7 (1-298) His

Source

E.coli

Bio Activity

Testing in progress.

Purity

>90% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.

Species

Human

Background

BIRC7, also known as Livin, is a member of the inhibitor of apoptosis protein (IAP) family. It plays a dual role as an apoptotic regulator, capable of exerting both proapoptotic and anti-apoptotic activities. Livin is crucial for controlling apoptosis, cell proliferation, and cell cycle regulation. Its anti-apoptotic function is primarily mediated through the inhibition of caspases, including CASP3, CASP7, and CASP9, as well as through its E3 ubiquitin-protein ligase activity. Livin promotes cell survival by ubiquitinating and targeting DIABLO/SMAC for degradation, thereby preventing DIABLO/SMAC from disrupting XIAP/BIRC4-caspase interactions. Livin protects cells from apoptosis induced by TNF or chemical agents such as adriamycin, etoposide, and staurosporine. This anti-apoptotic effect is achieved through the activation of MAPK8/JNK1 and possibly MAPK9/JNK2, which depends on TAB1 and MAP3K7/TAK1. In vitro, Livin inhibits CASP3 and the proteolytic activation of pro-CASP9. Structurally, Livin contains a single baculoviral IAP repeat (BIR) domain and a RING domain at the C-terminus. Overexpression of Livin has been observed in various cancers, including lung, colon, and prostate cancers, making it a potential therapeutic target.

Usage guide

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped with blue ice. Bulk packages of recombinant proteins are provided as frozen liquid which are shipped with dry ice.

Storage

Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.