Human IL-8/CXCL8 Tag Free Protein

Specification

20μg

50μg

100μg

Quota

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Catalog# HA210642

Human IL-8/CXCL8 Tag Free Protein

Product Information

Product name

Human IL-8/CXCL8 Tag Free Protein

Catalog No.

HA210642

Predicted molecular mass

9.2 kD

Protein construction description

A DNA sequence encoding the human IL-8/CXCL8 protein (P10145) (Glu 21-Ser 99) was expressed with tag free.

Accession

P10145

Protein construction

IL-8/CXCL8 (21-99)

Source

HEK293

Bio Activity

Measured in a cell proliferation assay using THP-1 human monocyte cells. The ED50 for this effect is 100-500 ng/mL.

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.

Species

Human

Background

Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection. Also plays an important role in neutrophil activation. Released in response to an inflammatory stimulus, exerts its effect by binding to the G-protein-coupled receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes and endothelial cells. G-protein heterotrimer (alpha, beta, gamma subunits) constitutively binds to CXCR1/CXCR2 receptor and activation by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in neutrophils) and activation of several downstream signaling pathways including PI3K and MAPK pathways.

Usage guide

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped with blue ice. Bulk packages of recombinant proteins are provided as frozen liquid which are shipped with dry ice.

Storage

Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.