Phospho-Histone H3 (T3) Recombinant Rabbit Monoclonal Antibody [JE42-48]

Quota

Contact Us

Email: [email protected]

Phone: 0571-88062880

Find Local Distributors

Catalog# HA721796

Phospho-Histone H3 (T3) Recombinant Rabbit Monoclonal Antibody [JE42-48]

Application

WB
IHC-P

Reactivity

Human
Mouse

Predicted reactivity

Overview

Product Name

Phospho-Histone H3 (T3) Recombinant Rabbit Monoclonal Antibody [JE42-48]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Thr3 of Human Histone H3.

Species Reactivity

Human, Mouse (Predicted: Rat)

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 15 kDa

Positive Control

HeLa treated with 20% FBS and 100nM Calyculin A for 30 minutes cell lysate, NIH/3T3 treated with 20% PBS and 100nM Calyculin A for 30 minutes cell lysate, mouse breast tissue, mouse skin tissue.

Conjugation

unconjugated

Clone Number

JE42-48

RRID

AB_3072908

Product Features

Form

Liquid

Concentration

1ug/ul

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

WB
1:1,000

IHC-P
1:200-1:1,000

Target

Function

Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Background References

1. Strobino M. et. al. Loss of histone H3.3 results in DNA replication defects and altered origin dynamics in C. elegans. Genome Res. 2020 Dec

2. Ince AT. et. al. The relationship of Serum Histone H3.3 and H4 with chronic Hepatitis B. J Pak Med Assoc. 2020 Sep

Subcellular Location

Nucleus, Chromosome.

UNIPROT

SwissProt: P68431 Human

SwissProt: P68433 Mouse

SwissProt: Q6LED0 Rat

Synonyms

HIST1 cluster, H3J antibody

Histone gene cluster 1, H3 histone family, member E antibody

Histone gene cluster 1, H3G antibody

H3 histone family, member J antibody

HIST1 cluster, H3E antibody

HIST1 cluster, H3I antibody

Histone gene cluster 1, H3C antibody

FLJ92264 antibody

H3 histone family, member A antibody

H3 histone family, member B antibody

Expand

Images

☑ Cell treatment (CT)

Western blot analysis of Phospho-Histone H3 (T3) on different lysates with Rabbit anti-Phospho-Histone H3 (T3) antibody (HA721796) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 20% FBS and 100nM Calyculin A for 30 minutes cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: NIH/3T3 treated with 20% PBS and 100nM Calyculin A for 30 minutes cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 15 kDa
Observed band size: 15 kDa

Exposure time: 25 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721796) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse breast tissue with Rabbit anti-Phospho-Histone H3 (T3) antibody (HA721796) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721796) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse skin tissue with Rabbit anti-Phospho-Histone H3 (T3) antibody (HA721796) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721796) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Phospho-Histone H3 (T3) Recombinant Rabbit Monoclonal Antibody [JE42-48]

Contact Us

Overview

Product Name

Antibody Type

Immunogen

Species Reactivity

Validated Applications

Molecular Weight

Positive Control

Conjugation

Clone Number

RRID

Product Features

Form

Concentration

Storage Instructions

Storage Buffer

Isotype

Purification Method

Application Dilution

Target

Function

Background References

Subcellular Location

UNIPROT

Synonyms

Images

Products with the same target and pathway

Histone H3 Recombinant Mouse Monoclonal Antibody [6-A7-R] - Loading control

Application: WB,IHC-P,IF-Tissue,ChIP

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

Histone H3 (acetyl K23) Recombinant Rabbit Monoclonal Antibody [JE46-39]

Application: WB,IF-Cell

Reactivity: Human,Mouse

Conjugate: unconjugated

Histone H3 (mono methyl K18) Recombinant Rabbit Monoclonal Antibody [SA42-07]

Application: WB,IF-Cell,IF-Tissue,IHC-P

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

Histone H3 Rabbit Polyclonal Antibody

Application: WB

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

Histone H3 (acetyl K9) Recombinant Rabbit Monoclonal Antibody [PSH04-47] - ChIP Grade

Application: WB,IF-Cell,IHC-P,IF-Tissue,FC,ChIP,Dot Blot,IP

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

Histone H3 (mono+di+tri methyl K36) Recombinant Rabbit Monoclonal Antibody [PSH07-14]

Application: WB,IHC-P,IF-Cell,FC,ChIP

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

Histone H3 (mono methyl K4) Recombinant Rabbit Monoclonal Antibody [PSH07-15]

Application: WB,IF-Cell,ChIP,Dot Blot

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

Histone H3 (mono methyl K9) Recombinant Rabbit Monoclonal Antibody [JE43-27]

Application: WB,IF-Cell,IHC-P,FC,ChIP,IF-Tissue,Dot Blot

Reactivity: Human,Mouse,Rat,Monkey

Conjugate: unconjugated

Histone H3 (acetyl K36) Recombinant Rabbit Monoclonal Antibody [JE77-32]

Application: WB,IHC-P,IF-Cell,ChIP

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

Histone H3 (acetyl K18) Rabbit Polyclonal Antibody

Application: WB,IHC-P,IF-Cell

Reactivity: Human,Mouse

Conjugate: unconjugated

Histone H3 Mouse Monoclonal Antibody [3-C4]

Application: WB,IHC-P,mIHC,IF-Tissue,ChIP

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

Histone H3 (acetyl K27) Mouse Monoclonal Antibody [A6D6]

Application: WB,IF-Cell,IHC-P,FC,ChIP

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

Histone H3 (di methyl K4) Rabbit Polyclonal Antibody

Application: WB,IF-Cell,IHC-P,FC,Dot Blot

Reactivity: Human,Mouse,Rat