VDAC1 Rabbit Polyclonal Antibody
Catalog# R1307-1
VDAC1 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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IHC-P
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Human
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Zebrafish
Predicted species support after-sales service
Overview
Product Name
VDAC1 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human VDAC1 aa 188-231.
Species Reactivity
Human (Predicted: Zebrafish)
Predicted species support after-sales service
Validated Applications
WB, IF-Cell, IHC-P
Molecular Weight
Predicted band size: 31 kDa
Positive Control
HeLa cell lysate, Jurkat cell lysate, A431 cell lysate, Raji cell lysate, HepG2 cell lysate, SW480 cell lysate, A549 cell lysate, human brain tissue, Hela.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1ug/ul
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:200
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IF-Cell
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1:200
Applications in Publications
Target
Function
Voltage-dependent anion-selective channel (VDAC1) (also referred to as porin, isoform 1) is a small protein, originally discovered in the outer membrane of mitochondria where it constitutes the major pore-forming protein. The porin protein VDAC1 allows to the outer-most membrane of the mitochondrion free permeability to low molecular-weight solutes. VDAC1 has been shown to co-immunoprecipitate with the anti-apoptotic protein Bcl-2 and suggested to be involved in forming the mitochondrial pore which releases cytochrome c during apoptosis.
Background References
1. "Influenza virus PB1-F2 protein induces cell death through mitochondrial ANT3 and VDAC1."Zamarin D., Garcia-Sastre A., Xiao X., Wang R., Palese P. PLoS Pathog. 1:40-54(2005)
2. "Solution structure of the integral human membrane protein VDAC-1 in detergent micelles." Hiller S., Garces R.G., Malia T.J., Orekhov V.Y., Colombini M., Wagner G. Science 321:1206-1210(2008)
Sequence Similarity
Belongs to the eukaryotic mitochondrial porin family.
Tissue Specificity
Heart, liver and skeletal muscle.
Post-translational Modification
Phosphorylation at Ser-193 by NEK1 promotes the open conformational state preventing excessive mitochondrial membrane permeability and subsequent apoptotic cell death after injury. Phosphorylation by the AKT-GSK3B axis stabilizes the protein probably by preventing ubiquitin-mediated proteasomal degradation.; Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.
Subcellular Location
Mitochondrial membrane, cell membrane
UNIPROT
Synonyms
N2441 antibody
OMP2 antibody
POR1 antibody
hVDAC1 antibody
MGC111064 antibody
Mitochondrial Porin antibody
Outer mitochondrial membrane protein porin 1 antibody
Plasmalemmal porin antibody
Porin 31HL antibody
Porin 31HM antibody
ExpandImages
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Western blot analysis of VDAC1 on different lysates with Rabbit anti-VDAC1 antibody (R1307-1) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: A431 cell lysate
Lane 4: Raji cell lysate
Lane 5: HepG2 cell lysate
Lane 6: SW480 cell lysate
Lane 7: A549 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 1 minute 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1307-1) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-VDAC1 rabbit polyclonal antibody.
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☑ Knockdown (KD)
Western blot analysis of VDAC1 on different lysates with Rabbit anti-VDAC1 antibody (R1307-1) at 1/1,000 dilution.
Lane 1: HEK293-si NT cell lysate
Lane 2: HEK293-si VDAC1#1(no heat) cell lysate
Lane 3: HEK293-si VDAC1#2(no heat) cell lysate
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
R1307-1 was shown to specifically react with VDAC1 in HEK293-si NT cells. No band were observed when HEK293-si VDAC1 samples were tested. HEK293-si NT and HEK293-si VDAC1 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (R1307-1, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at 4℃ overnight. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
ICC staining VDAC1 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
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Mitochondrial proteomic analysis reveals the regulation of energy metabolism and reactive oxygen species production in Clematis terniflora DC. leaves under high-level UV-B radiation followed by dark treatmenttreatment
Author: Tao, M., Zhu, W., Han, H., Liu, S., Liu, A., Li, S., Fu, H., & Tian, J.
PMID: 34923174
Journal: Journal Of Proteomics
Application: WB
Reactivity:
Publish date: 2022 Mar
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Citation
Alternative Products
VDAC1 Recombinant Rabbit Monoclonal Antibody [SA93-03]
Application: WB,IF-Cell,IHC-P,FC,IF-Tissue
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
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