Vimentin Rabbit Polyclonal Antibody

Function

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Research studies have shown that vimentin is present in sarcomas, but not carcinomas, and its expression is examined in conjunction with that of other markers to distinguish between the two. Vimentin's dynamic structural changes and spatial re-organization in response to extracellular stimuli help to coordinate various signaling pathways.

Background References

1. "Nucleotide sequence of the human vimentin gene and regulation of its transcription in tissues and cultured cells." Perreau J., Lilienbaum A., Vasseur M., Paulin D. Gene 62:7-16(1988)

2. "Stabilization of vimentin coil2 fragment via an engineered disulfide." Chernyatina A.A., Strelkov S.V. J. Struct. Biol. 177:46-53(2012)

Sequence Similarity

Belongs to the intermediate filament family.

Tissue Specificity

Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.

Post-translational Modification

Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33. Phosphorylated on tyrosine residues by SRMS.; O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.; S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

Subcellular Location

Cytoplasm, Cell membrane, Cytoskeleton, Intermediate filament, Membrane, Nucleus.

UNIPROT

SwissProt: P08670 Human

Synonyms

CTRCT30 antibody

Epididymis luminal protein 113 antibody

FLJ36605 antibody

HEL113 antibody

VIM antibody

VIME_HUMAN antibody

Vimentin antibody