TBR2 Recombinant Rabbit Monoclonal Antibody [PSH07-88] - BSA and Azide free
Catalog# HA751195
TBR2 Recombinant Rabbit Monoclonal Antibody [PSH07-88] - BSA and Azide free
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WB
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IHC-P
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IHC-Fr
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Human
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Mouse
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Cynomolgus monkey
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Pig
Predicted species support after-sales service
Overview
Product Name
TBR2 Recombinant Rabbit Monoclonal Antibody [PSH07-88] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human TBR2 aa 1-300.
Species Reactivity
Human, Mouse (Predicted: Cynomolgus monkey, Pig)
Predicted species support after-sales service
Validated Applications
WB, IHC-P, IHC-Fr
Molecular Weight
Predicted band size: 73 kDa
Positive Control
NK-92 cell lysate, Mouse embryo tissue lysate, E14.5 mouse embryonic brain tissue.
Conjugation
unconjugated
Clone Number
PSH07-88
Product Features
Form
Liquid
Concentration
1ug/ul
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:5,000
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IHC-Fr
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1:500
Target
Function
Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes.
Background References
1. Joulia E et al. Eomes-dependent mitochondrial regulation promotes survival of pathogenic CD4+ T cells during inflammation. J Exp Med. 2024 Feb.
2. Wong P et al. T-BET and EOMES sustain mature human NK cell identity and antitumor function. J Clin Invest. 2023 Jul.
Subcellular Location
Nucleus.
Synonyms
eomes
EOMES_HUMAN
Eomesodermin
Eomesodermin homolog
t box brain 2
T-box brain protein 2
T-brain-2
Tbr 2
TBR-2
Images
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Application: IHC-Fr
Species: Mouse
Site: E14.5 embryonic brain
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
Immunohistochemical analysis of paraffin-embedded E14.5 mouse embryonic brain tissue with Rabbit anti-TBR2 antibody (HA751195) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751195) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Western blot analysis of TBR2 on different lysates with Rabbit anti-TBR2 antibody (HA751195) at 1/1,000 dilution.
Lane 1: NK-92 cell lysate
Lane 2: HeLa cell lysate (negative)
Lysates/proteins at 30 µg/Lane.
Predicted band size: 73 kDa
Observed band size: 73/85 kDa
Exposure time: 14 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751195) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of TBR2 on mouse embryo tissue lysates with Rabbit anti-TBR2 antibody (HA751195) at 1/1,000 dilution.
Lysates/proteins at 30 µg/Lane.
Predicted band size: 73 kDa
Observed band size: 73/85 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751195) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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