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TBR2 Recombinant Rabbit Monoclonal Antibody [PSH07-88]

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Catalog# HA722918

TBR2 Recombinant Rabbit Monoclonal Antibody [PSH07-88]

Application

  • WB

  • IHC-P

  • IHC-Fr

Reactivity

  • Human

  • Mouse

Predicted reactivity

  • Cynomolgus monkey

  • Pig

Predicted species support after-sales service

Overview

Product Name

TBR2 Recombinant Rabbit Monoclonal Antibody [PSH07-88]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human TBR2 aa 1-300.

Species Reactivity

Human, Mouse (Predicted: Cynomolgus monkey, Pig)

Predicted species support after-sales service

Validated Applications

WB, IHC-P, IHC-Fr

Molecular Weight

Predicted band size: 73 kDa

Positive Control

NK-92 cell lysate, Mouse embryo tissue lysate, E14.5 mouse embryonic brain tissue.

Conjugation

unconjugated

Clone Number

PSH07-88

Product Features

Form

Liquid

Concentration

1ug/ul

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:5,000

  • IHC-Fr

  • 1:500

Target

Function

Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes.

Background References

1. Joulia E et al. Eomes-dependent mitochondrial regulation promotes survival of pathogenic CD4+ T cells during inflammation. J Exp Med. 2024 Feb.

2. Wong P et al. T-BET and EOMES sustain mature human NK cell identity and antitumor function. J Clin Invest. 2023 Jul.

Subcellular Location

Nucleus.

UNIPROT

SwissProt: O95936 Human

SwissProt: O54839 Mouse

Synonyms

eomes

EOMES_HUMAN

Eomesodermin

Eomesodermin homolog

t box brain 2

T-box brain protein 2

T-brain-2

Tbr 2

TBR-2

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: E14.5 embryonic brain <br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: E14.5 embryonic brain

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • Immunohistochemical analysis of paraffin-embedded E14.5 mouse embryonic brain tissue with Rabbit anti-TBR2 antibody (<a href="/products/HA722918" style="font-weight: bold;text-decoration: underline;">HA722918</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722918" style="font-weight: bold;text-decoration: underline;">HA722918</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded E14.5 mouse embryonic brain tissue with Rabbit anti-TBR2 antibody (HA722918) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722918) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of TBR2 on different lysates with Rabbit anti-TBR2 antibody (<a href="/products/HA722918" style="font-weight: bold;text-decoration: underline;">HA722918</a>) at 1/1,000 dilution.<br /><br />Lane 1: NK-92 cell lysate<br />Lane 2: HeLa cell lysate (negative)<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 73 kDa<br />Observed band size: 73/85 kDa<br /><br />Exposure time: 14 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722918" style="font-weight: bold;text-decoration: underline;">HA722918</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of TBR2 on different lysates with Rabbit anti-TBR2 antibody (HA722918) at 1/1,000 dilution.

    Lane 1: NK-92 cell lysate
    Lane 2: HeLa cell lysate (negative)

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 73 kDa
    Observed band size: 73/85 kDa

    Exposure time: 14 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722918) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of TBR2 on mouse embryo tissue lysates with Rabbit anti-TBR2 antibody (<a href="/products/HA722918" style="font-weight: bold;text-decoration: underline;">HA722918</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 73 kDa<br />Observed band size: 73/85 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722918" style="font-weight: bold;text-decoration: underline;">HA722918</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TBR2 on mouse embryo tissue lysates with Rabbit anti-TBR2 antibody (HA722918) at 1/1,000 dilution.

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 73 kDa
    Observed band size: 73/85 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722918) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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