TGF beta 1 Recombinant Rabbit Monoclonal Antibody [PD00-17] - BSA and Azide free
Catalog# HA750485
TGF beta 1 Recombinant Rabbit Monoclonal Antibody [PD00-17] - BSA and Azide free
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WB
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IHC-P
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IF-Tissue
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Human
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Mouse
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Rat
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Monkey
Overview
Product Name
TGF beta 1 Recombinant Rabbit Monoclonal Antibody [PD00-17] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide (KLH-coupled) corresponding to Human TGF-Beta 1 C-terminal.
Species Reactivity
Human, Mouse, Rat, Monkey
Validated Applications
WB, IHC-P, IF-Tissue
Molecular Weight
Predicted band size: 44 kDa
Positive Control
K-562 cell lysate, Raji cell lysate, Saos-2 cell lysate, 786-0 cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, L-929 cell lysate, C6 cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, rat spleen tissue, mouse spleen tissue.
Conjugation
unconjugated
Clone Number
PD00-17
Product Features
Form
Liquid
Concentration
1ug/ul
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000-1:5,000
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IHC-P
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1:200
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IF-Tissue
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1:200
Target
Function
Transforming growth factor beta 1 or TGF-β1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation, and apoptosis. In humans, TGF-β1 is encoded by the TGFB1 gene. TGF-β is a multifunctional set of peptides that controls proliferation, differentiation, and other functions in many cell types. TGF-β acts synergistically with transforming growth factor-alpha (TGF-α) in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGF-β activation and signaling may result in apoptosis. Many cells synthesize TGF-β and almost all of them have specific receptors for this peptide. TGF-β1, TGF-β2, and TGF-β3 all function through the same receptor signaling systems. TGF-β1 was first identified in human platelets as a protein with a molecular mass of 25 kilodaltons with a potential role in wound healing. It was later characterized as a large protein precursor (containing 390 amino acids) that was proteolytically processed to produce a mature peptide of 112 amino acids. TGF-β1 plays an important role in controlling the immune system, and shows different activities on different types of cell, or cells at different developmental stages. Most immune cells (or leukocytes) secrete TGF-β1.
Background References
1. Takahara T. et. al.TGFB1 mRNA expression is associated with poor prognosis and specific features of inflammation in ccRCC. Virchows Arch. 2022 Feb
2. Abdel Mouti M. et. al. TGFB1/INHBA Homodimer/Nodal-SMAD2/3 Signaling Network: A Pivotal Molecular Target in PDAC Treatment. Mol Ther. 2021 Mar
Subcellular Location
Extracellular matrix, Secreted.
Synonyms
Cartilage-inducing factor antibody
CED antibody
Differentiation inhibiting factor antibody
DPD1 antibody
LAP antibody
Latency-associated peptide antibody
Prepro transforming growth factor beta 1 antibody
TGF beta 1 antibody
TGF beta antibody
TGF beta 1 protein antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of TGF beta 1 on different lysates with Rabbit anti-TGF beta 1 antibody (HA750485) at 1/5,000 dilution.
Lane 1: K-562 cell lysate
Lane 2: Raji cell lysate
Lane 3: Saos-2 cell lysate
Lane 4: 786-0 cell lysate
Lane 5: COS-1 cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: RAW264.7 cell lysate
Lane 8: L-929 cell lysate
Lane 9: C6 cell lysate
Lane 10: Mouse spleen tissue lysate
Lane 11: Mouse skeletal muscle tissue lysate (negative)
Lane 12: Rat spleen tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 44 kDa
Observed band size: 48/13 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750485) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-TGF beta 1 antibody (HA750485) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750485) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-TGF beta 1 antibody (HA750485) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750485) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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