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TGF beta 1 Recombinant Rabbit Monoclonal Antibody [PD00-17]

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Catalog# HA721143

TGF beta 1 Recombinant Rabbit Monoclonal Antibody [PD00-17]

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

TGF beta 1 Recombinant Rabbit Monoclonal Antibody [PD00-17]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide (KLH-coupled) corresponding to Human TGF-Beta 1 C-terminal.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 44 kDa

Positive Control

Raji cell lysate, K-562 cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, A549 cell lysate, L-929 cell lysate, NIH/3T3 cell lysate, rat spleen tissue, mouse spleen tissue.

Conjugation

unconjugated

Clone Number

PD00-17

RRID

AB_3072265

Product Features

Form

Liquid

Concentration

1ug/ul

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:200

  • IF-Tissue

  • 1:200

Target

Function

Transforming growth factor beta 1 or TGF-β1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation, and apoptosis. In humans, TGF-β1 is encoded by the TGFB1 gene. TGF-β is a multifunctional set of peptides that controls proliferation, differentiation, and other functions in many cell types. TGF-β acts synergistically with transforming growth factor-alpha (TGF-α) in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGF-β activation and signaling may result in apoptosis. Many cells synthesize TGF-β and almost all of them have specific receptors for this peptide. TGF-β1, TGF-β2, and TGF-β3 all function through the same receptor signaling systems. TGF-β1 was first identified in human platelets as a protein with a molecular mass of 25 kilodaltons with a potential role in wound healing. It was later characterized as a large protein precursor (containing 390 amino acids) that was proteolytically processed to produce a mature peptide of 112 amino acids. TGF-β1 plays an important role in controlling the immune system, and shows different activities on different types of cell, or cells at different developmental stages. Most immune cells (or leukocytes) secrete TGF-β1.

Background References

1. Takahara T. et. al.TGFB1 mRNA expression is associated with poor prognosis and specific features of inflammation in ccRCC. Virchows Arch. 2022 Feb

2. Abdel Mouti M. et. al. TGFB1/INHBA Homodimer/Nodal-SMAD2/3 Signaling Network: A Pivotal Molecular Target in PDAC Treatment. Mol Ther. 2021 Mar

Subcellular Location

Extracellular matrix, Secreted.

UNIPROT

SwissProt: P01137 Human

SwissProt: P04202 Mouse

SwissProt: P17246 Rat

Synonyms

Cartilage-inducing factor antibody

CED antibody

Differentiation inhibiting factor antibody

DPD1 antibody

LAP antibody

Latency-associated peptide antibody

Prepro transforming growth factor beta 1 antibody

TGF beta 1 antibody

TGF beta antibody

TGF beta 1 protein antibody

Expand

Images

  • Western blot analysis of TGF beta 1 on different lysates with Rabbit anti-TGF beta 1 antibody (<a href="/products/HA721143" style="font-weight: bold;text-decoration: underline;">HA721143</a>) at 1/2,000 dilution.<br /><br />Lane 1: Raji cell lysate (15 µg/Lane)<br />Lane 2: K-562 cell lysate (15 µg/Lane)<br />Lane 3: Mouse spleen tissue lysate (20 µg/Lane)<br />Lane 4: Rat spleen tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 44 kDa<br />Observed band size: 44 kDa<br /><br />Exposure time: 3 minutes 54 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721143" style="font-weight: bold;text-decoration: underline;">HA721143</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TGF beta 1 on different lysates with Rabbit anti-TGF beta 1 antibody (HA721143) at 1/2,000 dilution.

    Lane 1: Raji cell lysate (15 µg/Lane)
    Lane 2: K-562 cell lysate (15 µg/Lane)
    Lane 3: Mouse spleen tissue lysate (20 µg/Lane)
    Lane 4: Rat spleen tissue lysate (20 µg/Lane)

    Predicted band size: 44 kDa
    Observed band size: 44 kDa

    Exposure time: 3 minutes 54 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721143) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of TGF beta 1 on different lysates with Rabbit anti-TGF beta 1 antibody (<a href="/products/HA721143" style="font-weight: bold;text-decoration: underline;">HA721143</a>) at 1/1,000 dilution.<br /><br />Lane 1: Raji cell lysate<br />Lane 2: A549 cell lysate<br />Lane 3: L-929 cell lysate<br />Lane 4: NIH/3T3 cell lysate<br />Lane 5: Mouse spleen tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 44 kDa<br />Observed band size: 48/13 kDa<br /><br />Exposure time: 2 minutes 50 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721143" style="font-weight: bold;text-decoration: underline;">HA721143</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TGF beta 1 on different lysates with Rabbit anti-TGF beta 1 antibody (HA721143) at 1/1,000 dilution.

    Lane 1: Raji cell lysate
    Lane 2: A549 cell lysate
    Lane 3: L-929 cell lysate
    Lane 4: NIH/3T3 cell lysate
    Lane 5: Mouse spleen tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 44 kDa
    Observed band size: 48/13 kDa

    Exposure time: 2 minutes 50 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721143) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-TGF beta 1 antibody (<a href="/products/HA721143" style="font-weight: bold;text-decoration: underline;">HA721143</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721143" style="font-weight: bold;text-decoration: underline;">HA721143</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-TGF beta 1 antibody (HA721143) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721143) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-TGF beta 1 antibody (<a href="/products/HA721143" style="font-weight: bold;text-decoration: underline;">HA721143</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721143" style="font-weight: bold;text-decoration: underline;">HA721143</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-TGF beta 1 antibody (HA721143) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721143) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Filter:
  1. IF
  2. WB
  3. FC
  4. IHC

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Conjugate: unconjugated

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