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HA tag Rabbit Polyclonal Antibody

Specification

Quota

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BIO-STATION LIMITED

Contact: Sanry CHEN

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Phone: 852-35650223

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Catalog# 0906-1

HA tag Rabbit Polyclonal Antibody

Application

  • WB

  • IP

Reactivity

  • Species independent

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

HA tag Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide immune sequence is YPYDVPDYAC.

Species Reactivity

Species independent

Validated Applications

WB, IP

Positive Control

C-terminal HA-tagged recombinant protein, N-terminal HA-tagged recombinant protein.

Conjugation

unconjugated

RRID

AB_3068712

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:5,000-1:10,000

  • IP

  • 2-5 µg/ml.

Target

Function

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106. This antibody is used to detect proteins that are tagged with HA epitope (YPYDVPDYA) and are expressed in prokaryotic and eukaryotic cells.

Background References

1. Cao, H., Yang, C., Quan, S., Hu, T., Zhang, L., Zhang, Y., Liu, Q. (2018). Novel T3SS effector EseK in Edwardsiella piscicida is chaperoned by EscH and EscS to express virulence. Cell Microbiol, 20(1). PMID: 29024267

2. Zhou, Y., Xiong, L., Zhang, Y., Yu, R., Jiang, X., & Xu, G. (2016). Quantitative proteomics identifies myoferlin as a novel regulator of A Disintegrin and Metalloproteinase 12 in HeLa cells. J Proteomics, 148, 94-104. PMID: 27432471

3. Wang, K., Jin, X., Chen, Y., Song, Z., Jiang, X., Hu, F., Topping, D. L. (2016). Polyphenol-Rich Propolis Extracts Strengthen Intestinal Barrier Function by Activating AMPK and ERK Signaling. Nutrients, 8(5).PMID: 27164138

4. Fu, G., Xu, Q., Qiu, Y., Jin, X., Xu, T., Dong, S., Lu, L. (2017). Suppression of Th17 cell differentiation by misshapen/NIK-related kinase MINK1. J Exp Med, 214(5), 1453-1469. PMID: 28400474

5. You, Q., Wu, Y., Yao, N., Shen, G., Zhang, Y., Xu, L., Ju, C. (2015). Interaction of AIM with insulin-like growth factor-binding protein-4. Int J Mol Med, 36(3), 833-838.

Synonyms

HA Tag

Images

  • Western blot analysis of HA tag on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/0906-1" style="font-weight: bold;text-decoration: underline;">0906-1</a>, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.<br />Positive control:<br />Lane 1: C-terminal HA-tagged recombinant protein<br />Lane 2: N-terminal HA-tagged recombinant protein

    Western blot analysis of HA tag on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0906-1, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: C-terminal HA-tagged recombinant protein
    Lane 2: N-terminal HA-tagged recombinant protein

  • HA tag was immunoprecipitated in 5µg C terminal HA Tag fusion protein lysate with <a href="/products/0906-1" style="font-weight: bold;text-decoration: underline;">0906-1</a> at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using <a href="/products/M1008-1" style="font-weight: bold;text-decoration: underline;">M1008-1</a> at 1/1000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/20,000 dilution was used for 60 mins at room temperature.<br /><br />Lane 1: HA Tag fusion protein lysate (input).<br />Lane 2: <a href="/products/ET1611-49" style="font-weight: bold;text-decoration: underline;">ET1611-49</a> IP in HA Tag fusion protein lysate.<br />Lane 3: <a href="/products/0906-1" style="font-weight: bold;text-decoration: underline;">0906-1</a> IP in HA Tag fusion protein lysate.<br />Lane 4: Rabbit IgG instead of <a href="/products/0906-1" style="font-weight: bold;text-decoration: underline;">0906-1</a> in HA Tag fusion protein lysate.<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST

    HA tag was immunoprecipitated in 5µg C terminal HA Tag fusion protein lysate with 0906-1 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using M1008-1 at 1/1000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/20,000 dilution was used for 60 mins at room temperature.

    Lane 1: HA Tag fusion protein lysate (input).
    Lane 2: ET1611-49 IP in HA Tag fusion protein lysate.
    Lane 3: 0906-1 IP in HA Tag fusion protein lysate.
    Lane 4: Rabbit IgG instead of 0906-1 in HA Tag fusion protein lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Alternative Products

metafield image

HA tag Recombinant Rabbit Monoclonal Antibody [SN07-06]

Application: WB,IP

Reactivity: Species independent

Conjugate: unconjugated

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