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CD31 Rabbit Polyclonal Antibody

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Catalog# ER31219

CD31 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • FC

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

CD31 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within C-terminal residues of CD31.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, FC, IHC-P

Molecular Weight

Predicted band size: 83 kDa

Positive Control

THP-1 cell lysate, Mouse lung tissue lysate, Rat lung tissue lysate, human lung tissue, human tonsil tissue, rat lung tissue, Hela, HUVEC, NIH/3T3, SW480, Jurkat.

Conjugation

unconjugated

RRID

AB_3069567

Product Features

Form

Liquid

Concentration

1ug/ul

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:200

  • FC

  • 1:100-1:200

  • IHC-P

  • 1:1,000-1:5,000

Target

Function

PECAM-1 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte transmigration, angiogenesis, and integrin activation. CD31 is also expressed in certain tumors, including epithelioid hemangioendothelioma, epithelioid sarcoma-like hemangioendothelioma, other vascular tumors, histiocytic malignancies, and plasmacytomas. It is rarely found in some sarcomas, such as Kaposi's sarcoma and carcinomas. In immunohistochemistry, CD31 is used primarily to demonstrate the presence of endothelial cells in histological tissue sections. This can help to evaluate the degree of tumour angiogenesis, which can imply a rapidly growing tumor

Background References

1. Brown S et al. Apoptosis disables CD31-mediated cell detachment from phagocytes promoting binding and engulfment. Nature 418:200-203 (2002).

2. Sardjono C T et al. Palmitoylation at Cys595 is essential for PECAM-1 localisation into membrane microdomains and for efficient PECAM-1-mediated cytoprotection.Thromb Haemost 96(6):756-66 (2006).

3. Yeh J C et al. Regulation of G protein-coupled receptor activities by the platelet-endothelial cell adhesion molecule, PECAM-1. Biochemistry

Tissue Specificity

Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Expressed in human umbilical vein endothelial cells (HUVECs) (at protein level). Expressed on neutrophils (at protein level). Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U-937 histiocytic lymphoma cell lines (at protein level).

Post-translational Modification

Phosphorylated on Ser and Tyr residues after cellular activation by src kinases. Upon activation, phosphorylated on Ser-729 which probably initiates the dissociation of the membrane-interaction segment (residues 709-729) from the cell membrane allowing the sequential phosphorylation of Tyr-713 and Tyr-690. Constitutively phosphorylated on Ser-734 in resting platelets. Phosphorylated on tyrosine residues by FER and FES in response to FCER1 activation (By similarity). In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.; Palmitoylation by ZDHHC21 is necessary for cell surface expression in endothelial cells and enrichment in membrane rafts.

Subcellular Location

Cell membrane, Cell junction.

UNIPROT

SwissProt: P16284 Human

SwissProt: Q08481 Mouse

SwissProt: Q3SWT0 Rat

Synonyms

Adhesion molecule antibody

CD31 antibody

CD31 antigen antibody

CD31 EndoCAM antibody

EndoCAM antibody

FLJ34100 antibody

FLJ58394 antibody

GPIIA antibody

GPIIA' antibody

PECA1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of CD31 on different lysates with Rabbit anti-CD31 antibody (<a href="/products/ER31219" style="font-weight: bold;text-decoration: underline;">ER31219</a>) at 1/1,000 dilution.<br /><br />Lane 1: THP-1 cell lysate (10 µg/Lane)<br />Lane 2: NIH/3T3 cell lysate (negative) (10 µg/Lane)<br />Lane 3: Mouse lung tissue lysate (40 µg/Lane)<br />Lane 4: Rat lung tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 83 kDa<br />Observed band size: 120-130 kDa<br /><br />Exposure time: Lane 1: 16 seconds; Lane 2-4: 1 minute 2 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ER31219" style="font-weight: bold;text-decoration: underline;">ER31219</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of CD31 on different lysates with Rabbit anti-CD31 antibody (ER31219) at 1/1,000 dilution.

    Lane 1: THP-1 cell lysate (10 µg/Lane)
    Lane 2: NIH/3T3 cell lysate (negative) (10 µg/Lane)
    Lane 3: Mouse lung tissue lysate (40 µg/Lane)
    Lane 4: Rat lung tissue lysate (40 µg/Lane)

    Predicted band size: 83 kDa
    Observed band size: 120-130 kDa

    Exposure time: Lane 1: 16 seconds; Lane 2-4: 1 minute 2 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER31219) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-CD31 antibody (<a href="/products/ER31219" style="font-weight: bold;text-decoration: underline;">ER31219</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER31219" style="font-weight: bold;text-decoration: underline;">ER31219</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-CD31 antibody (ER31219) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER31219) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD31 antibody (<a href="/products/ER31219" style="font-weight: bold;text-decoration: underline;">ER31219</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER31219" style="font-weight: bold;text-decoration: underline;">ER31219</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD31 antibody (ER31219) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER31219) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-CD31 antibody (<a href="/products/ER31219" style="font-weight: bold;text-decoration: underline;">ER31219</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER31219" style="font-weight: bold;text-decoration: underline;">ER31219</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-CD31 antibody (ER31219) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER31219) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ICC staining CD31 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining CD31 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining CD31 in HUVEC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining CD31 in HUVEC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining CD31 in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining CD31 in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining CD31 in SW480 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining CD31 in SW480 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Flow cytometric analysis of Jurkat cells with CD31 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.

    Flow cytometric analysis of Jurkat cells with CD31 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Filter:
  1. IHC
  2. IF
  3. WB
  4. IHC-P
  5. ICC

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