CD11b Mouse Monoclonal Antibody [4-6]
Catalog# EM1701-42
CD11b Mouse Monoclonal Antibody [4-6]
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IHC-P
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IF-Cell
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FC
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WB
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IF-Tissue
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Human
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unconjugated
Overview
Product Name
CD11b Mouse Monoclonal Antibody [4-6]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human CD11b aa 544-743 / 1,152.
Species Reactivity
Human
Validated Applications
IHC-P, IF-Cell, FC, WB, IF-Tissue
Target Molecular Weight
Predicted band size: 127 kDa
Positive Control
TF-1 cell lysate, THP-1 cell lysate, U-937 cell lysate, A431, LOVO, human tonsil tissue, human spleen tissue.
Conjugation
unconjugated
Clone Number
4-6
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG2b
Purification Method
Protein G affinity purified.
Application Dilution
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
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WB
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1:1,000
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IF-Tissue
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1:50
Target
Function
Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration. In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils. May regulate phagocytosis-induced apoptosis in extravasated neutrophils. May play a role in mast cell development.
Background References
1. Jerke U et al.Complement receptor Mac-1 is an adaptor for NB1 (CD177)-mediated PR3-ANCA neutrophil activation. J. Biol. Chem. 286:7070-7081 (2011).
2. Bai M. et al. CD177 modulates human neutrophil migration through activation-mediated integrin and chemoreceptor regulation. Blood 0:0-0 (2017).
Sequence Similarity
Belongs to the integrin alpha chain family.
Tissue Specificity
Predominantly expressed in monocytes and granulocytes. Expressed in neutrophils (at protein level).
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
antigen CD11b (p170) antibody
Antigen CD11b p170 antibody
CD11 antigen like family member B antibody
CD11 antigen-like family member B antibody
CD11b antibody
CD11b/CD18 antibody
CD49d antibody
Cell surface glycoprotein MAC-1 subunit alpha antibody
Complement component 3 receptor 3 subunit antibody
Complement Component Receptor 3 Alpha antibody
ExpandImages
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Western blot analysis of CD11b on different lysates with Mouse anti-CD11b antibody (EM1701-42) at 1/1,000 dilution.
Lane 1: TF-1 cell lysate
Lane 2: THP-1 cell lysate
Lane 3: U-937 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 127 kDa
Observed band size: 170 kDa
Exposure time: 30 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-42) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
ICC staining CD11b (green) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining CD11b (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD11b antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD11b antibody. Counter stained with hematoxylin.
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Application: IF-Tissue
Species: Human
Site: Spleen
Sample: Paraffin-embedded section
Antibody concentration: 1/50
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
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Optimized CAR-T therapy based on spatiotemporal changes and chemotactic mechanisms of MDSCs induced by hypofractionated radiotherapy
Journal: Molecular Therapy
DOI:
IF: 12.4
Application: FC
Reactivity: Mouse
Publish date: 2023 Jul
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