PMS2 Recombinant Rabbit Monoclonal Antibody [SY08-09]
Catalog# ET1605-1
PMS2 Recombinant Rabbit Monoclonal Antibody [SY08-09]
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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IP
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FC
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Human
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unconjugated
Overview
Product Name
PMS2 Recombinant Rabbit Monoclonal Antibody [SY08-09]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human PMS2 aa 1-50 / 862.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IF-Tissue, IHC-P, IP, FC
Target Molecular Weight
Predicted band size: 96 kDa
Positive Control
HeLa, HeLa cell lysate, Jurkat cell lysate, HepG2 cell lysate, U-2 OS cell lysate, human breast cancer tissue, human breast ductal carcinoma tissue, human tonsil tissue, HeLa.
Conjugation
unconjugated
Clone Number
SY08-09
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000-1:5,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:200
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IHC-P
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1:50-1:200
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IP
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Use at an assay dependent concentration.
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FC
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1:1,000
Target
Function
Mismatch repair endonuclease PMS2 (postmeiotic segregation increased 2) is an enzyme that in humans is encoded by the PMS2 gene. This gene is one of the PMS2 gene family members, which are found in clusters on chromosome 7. Human PMS2-related genes are located at bands 7p12, 7p13, 7q11, and 7q22. Exons 1 through 5 of these homologues share high degree of identity to human PMS2. The product of this gene is involved in DNA mismatch repair. The protein forms a heterodimer with MLH1, and this complex interacts with MSH2 bound to mismatched bases. Defects in this gene are associated with hereditary nonpolyposis colorectal cancer, with Turcot syndrome, and are a cause of supratentorial primitive neuroectodermal tumors. Alternatively spliced transcript variants have been observed.
Background References
1. Andini KD et al. PMS2-associated Lynch syndrome: Past, present and future. Front Oncol. 2023 Feb
2. Chen J et al. PMS2 amplification contributes brain metastasis from lung cancer. Biol Proced Online. 2024 May
Sequence Similarity
Belongs to the DNA mismatch repair MutL/HexB family.
Subcellular Location
Nucleus.
UNIPROT
Synonyms
DNA mismatch repair gene homologue antibody
DNA mismatch repair protein PMS2 antibody
H_DJ0042M02.9 antibody
HNPCC4 antibody
Mismatch repair endonuclease PMS2 antibody
Mismatch repair gene PMSL2 antibody
PMS 2 antibody
PMS1 protein homolog 2 antibody
PMS2 antibody
PMS2 postmeiotic segregation increased 2 antibody
ExpandImages
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☑ Relative expression (RE)
Immunocytochemistry analysis of HeLa (positive) and HCT 116 (negative) cells labeling PMS2 with Rabbit anti-PMS2 antibody (ET1605-1) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PMS2 antibody (ET1605-1) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
☑ Relative expression (RE)
Western blot analysis of PMS2 on different lysates with Rabbit anti-PMS2 antibody (ET1605-1) at 1/10,000 dilution and competitor's antibody at 1/10,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: HCT 116 cell lysate (negative) (20 µg/Lane)
Lane 4: HepG2 cell lysate (20 µg/Lane)
Predicted band size: 96 kDa
Observed band size: 120 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-1) at 1/10,000 dilution and competitor's antibody at 1/10,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Relative expression (RE)
Western blot analysis of PMS2 on different lysates with Rabbit anti-PMS2 antibody (ET1605-1) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: U-2 OS cell lysate
Lane 3: Jurkat cell lysate
Lane 4: HepG2 cell lysate
Lane 5: HCT 116 cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 96 kDa
Observed band size: 120 kDa
Exposure time: 37 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-1) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of PMS2 on different lysates with Rabbit anti-PMS2 antibody (ET1605-1) at 1/5,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-PMS2 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 96 kDa
Observed band size: 100 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-1) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-PMS2 antibody (ET1605-1) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-1) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-PMS2 antibody (ET1605-1) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-1) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-PMS2 antibody (ET1605-1) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-1) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human breast ductal carcinoma tissue with Rabbit anti-PMS2 antibody (ET1605-1) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-1) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-PMS2 antibody (ET1605-1) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-1) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Flow cytometric analysis of HeLa (positive, red) and HCT 116 (negative, green) cells labeling PMS2.
Cells were fixed and permeabilized. Then stained with the primary antibody (ET1605-1, red) at 1/1,000 dilution and competitor's antibody (red) at 1/200 dilution, compared with Rabbit IgG Isotype Control (HeLa black, HCT 116 light green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"