Histone H3 Rabbit Polyclonal Antibody

Specification

50μl

100μl

Quota

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BIO-STATION LIMITED

Contact: Sanry CHEN

Email: info@bio-station.net

Phone: 852-35650223

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Catalog# HA500298

Histone H3 Rabbit Polyclonal Antibody

Application

Reactivity

Zebrafish
Human
Mouse

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Histone H3 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within Zebrafish Histone H3 aa 1-50 / 136.

Species Reactivity

Zebrafish, Human, Mouse

Validated Applications

Molecular Weight

Predicted band size: 15 kDa

Positive Control

Zebrafish tissue lysates.

Conjugation

unconjugated

RRID

AB_3071393

Product Features

Form

Liquid

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

WB
1:500-1:5,000

Applications in Publications

WB	See 1 publications below

Species in Publications

Human

See 1 publications below

Target

Function

Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Background References

1. Jacob Y.et.al.Selective methylation of histone H3 variant H3.1 regulates heterochromatin replication.Science 343:1249-1253(2014).

Subcellular Location

Nucleus,Chromosome.

UNIPROT

SwissProt: Q6PI20 Zebrafish

SwissProt: Q4QRF4 Zebrafish

SwissProt: B2GSF5 Zebrafish

Synonyms

H3 histone family member E pseudogene antibody

H3 histone family, member A antibody

H3/A antibody

H31_HUMAN antibody

H3F3 antibody

H3FA antibody

Hist1h3a antibody

HIST1H3B antibody

HIST1H3C antibody

HIST1H3D antibody

Expand

Images

Western blot analysis of Histone H3 on zebrafish tissue lysates with Rabbit anti-Histone H3 antibody (HA500298) at 1/5,000 dilution.

Lysates/proteins at 10 µg/Lane.

Predicted band size: 15 kDa
Observed band size: 15 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500298) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Filter:

Linear ubiquitination regulates the KSHV replication and transcription activator protein to control infection

Author: Luan Yi,et al

PMID: 38951495
Journal: Nature Communications
- Application: WB
- Reactivity: Human
- Publish date: 2024 Jul
- Citation

Histone H3 Rabbit Polyclonal Antibody

Specification

Contact Us

BIO-STATION LIMITED

Overview

Product Name

Antibody Type

Immunogen

Species Reactivity

Validated Applications

Molecular Weight

Positive Control

Conjugation

RRID

Product Features

Form

Storage Instructions

Storage Buffer

Isotype

Purification Method

Application Dilution

Applications in Publications

Species in Publications

Target

Function

Background References

Subcellular Location

UNIPROT

Synonyms

Images

Citation

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