TPH2 Recombinant Rabbit Monoclonal Antibody [PSH04-89]
Catalog# HA722195
TPH2 Recombinant Rabbit Monoclonal Antibody [PSH04-89]
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WB
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IHC-P
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IHC-Fr
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IP
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Mouse
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Rat
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Cynomolgus monkey
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Pig
Predicted species support after-sales service
Overview
Product Name
TPH2 Recombinant Rabbit Monoclonal Antibody [PSH04-89]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein with Human THP2 aa 1-490.
Species Reactivity
Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
Predicted species support after-sales service
Validated Applications
WB, IHC-P, IHC-Fr, IP
Molecular Weight
Predicted band size: 56 kDa
Positive Control
Mouse hippocampus tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, rat hippocampus tissue lysate, mouse midbrain tissue, mouse cerebrum tissue, rat cerebrum tissue, mouse brain (raphe nucleus) tissue.
Conjugation
unconjugated
Clone Number
PSH04-89
Product Features
Form
Liquid
Concentration
1ug/ul
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IHC-P
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1:500
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IHC-Fr
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1:500
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IP
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1-2μg/sample
Target
Function
Tryptophan hydroxylase 2 (TPH2) is an isozyme of tryptophan hydroxylase found in vertebrates. In humans, TPH2 is primarily expressed in the serotonergic neurons of the brain, with the highest expression in the raphe nucleus of the midbrain. Until the discovery of TPH2 in 2003, serotonin levels in the central nervous system were believed to be regulated by serotonin synthesis in peripheral tissues, in which tryptophan hydroxylase is the dominant form.
Background References
1. Zaniewska M et al. Tph2 Gene Expression Defines Ethanol Drinking Behavior in Mice. Cells. 2022 Mar
2. Liu H et al. TPH2 in the Dorsal Raphe Nuclei Regulates Energy Balance in a Sex-Dependent Manner. Endocrinology. 2021 Jan
Subcellular Location
Cytosol, neuron projection.
Synonyms
MGC138871 antibody
ADHD7 antibody
FLJ37295 antibody
MGC138872 antibody
Neuronal tryptophan hydroxylase antibody
NTPH antibody
TPH 2 antibody
Tph2 antibody
TPH2_HUMAN antibody
Tryptophan 5-hydroxylase 2 antibody
ExpandImages
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Application: IHC-Fr
Species: Mouse
Site: Raphe nucleus
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
Immunohistochemical analysis of paraffin-embedded mouse midbrain tissue with Rabbit anti-TPH2 antibody (HA722195) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722195) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue with Rabbit anti-TPH2 antibody (HA722195) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722195) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue with Rabbit anti-TPH2 antibody (HA722195) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722195) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Western blot analysis of TPH2 on different lysates with Rabbit anti-TPH2 antibody (HA722195) at 1/2,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Mouse kidney tissue lysate (negative)
Lane 3: Rat brain tissue lysate
Lane 4: Rat kidney tissue lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 56 kDa
Observed band size: 50 kDa
Exposure time: 1 minute 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722195) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
TPH2 was immunoprecipitated from 0.2 mg rat brain tissue lysate with HA722195 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA722195 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: rat brain tissue lysate (input)
Lane 2: HA722195 IP in rat brain tissue lysate
Lane 3: Rabbit IgG instead of HA722195 in rat brain tissue lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 3 minutes; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"