Presenilin 2 / AD5 Recombinant Rabbit Monoclonal Antibody [PSH05-18]
Catalog# HA722243
Presenilin 2 / AD5 Recombinant Rabbit Monoclonal Antibody [PSH05-18]
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WB
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IP
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Human
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Mouse
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Rat
Overview
Product Name
Presenilin 2 / AD5 Recombinant Rabbit Monoclonal Antibody [PSH05-18]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Presenilin 2 aa 300-400.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IP
Molecular Weight
Predicted band size: 50 kDa
Positive Control
HeLa cell lysate, HepG2 cell lysate, A549 cell lysate, 293T cell lysate, SH-SY5Y cell lysate, RAW264.7 cell lysate, Neuro-2a cell lysate, C2C12 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, C6 cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat liver tissue lysate.
Conjugation
unconjugated
Clone Number
PSH05-18
Product Features
Form
Liquid
Concentration
1ug/ul
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IP
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1-2μg/sample
Target
Function
Presenilin-2 is a protein that (in humans) is encoded by the PSEN2 gene. Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1; PSEN2) or the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor, such that they either directly regulate gamma-secretase activity or themselves are protease enzymes. Two alternative transcripts of PSEN2 have been identified. In melanocytic cells PSEN2 gene expression may be regulated by MITF.
Background References
1. Barazzuol L et al. Unraveling Presenilin 2 Functions in a Knockout Zebrafish Line to Shed Light into Alzheimer\'s Disease Pathogenesis. Cells. 2023 Jan
2. Nam H et al. Presenilin 2 N141I mutation induces hyperactive immune response through the epigenetic repression of REV-ERBalpha. Nat Commun. 2022 Apr
Subcellular Location
Endoplasmic reticulum membrane, Golgi apparatus membrane.
Synonyms
AD3L antibody
AD3LP antibody
AD4 antibody
AD5 antibody
Alzheimer disease 4 antibody
CMD1V antibody
E5-1 antibody
OTTHUMP00000035671 antibody
OTTHUMP00000035672 antibody
OTTHUMP00000228286 antibody
ExpandImages
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Western blot analysis of Presenilin 2 / AD5 on different lysates with Rabbit anti-Presenilin 2 / AD5 antibody (HA722243) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: A549 cell lysate (20 µg/Lane)
Lane 4: 293T cell lysate (20 µg/Lane)
Lane 5: SH-SY5Y cell lysate (20 µg/Lane)
Lane 6: RAW264.7 cell lysate (20 µg/Lane)
Lane 7: Neuro-2a cell lysate (20 µg/Lane)
Lane 8: C2C12 cell lysate (20 µg/Lane)
Lane 9: NIH/3T3 cell lysate (20 µg/Lane)
Lane 10: PC-12 cell lysate (20 µg/Lane)
Lane 11: C6 cell lysate (20 µg/Lane)
Lane 12: Human brain tissue lysate (40 µg/Lane)
Lane 13: Mouse brain tissue lysate (40 µg/Lane)
Lane 14: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 50 kDa
Observed band size: 23 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722243) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Presenilin 2 / AD5 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA722243 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA722243 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (input)
Lane 2: HA722243 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of HA722243 in HeLa cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 59 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"