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Symmetric Di-Methyl-Histone H3 (R17)
Symmetric Di-Methyl-Histone H3 (R17) Recombinant Rabbit Monoclonal Antibody [PSH27-44]

Symmetric Di-Methyl-Histone H3 (R17) Recombinant Rabbit Monoclonal Antibody [PSH27-44]

DATA SHEET
MSDS
COA

Quota

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BIO-STATION LIMITED

Contact: Sanry CHEN

Email: info@bio-station.net

Phone: 852-35650223

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Catalog# HA724695

Symmetric Di-Methyl-Histone H3 (R17) Recombinant Rabbit Monoclonal Antibody [PSH27-44]

Application

WB
IF-Cell

Reactivity

Human
Mouse
Rat

Conjugation

unconjugated

Safety datasheet

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MSDS_HUABIO.pdf
MSDS_HUABIO.pdf
MSDS_HA724695_Europe.pdf
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Overview

Product Name

Symmetric Di-Methyl-Histone H3 (R17) Recombinant Rabbit Monoclonal Antibody [PSH27-44]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell

Target Molecular Weight

Predicted band size: 15 kDa

Positive Control

293T(Human embryonic kidney cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate, C6 (Rat glioma cell) cell lysate.

Conjugation

unconjugated

Clone Number

PSH27-44

Product Features

Form

Liquid

Concentration

Lot Concentration Lookup

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

WB
1:500

IF-Cell
1:50

Target

Function

Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fibers. Two molecules of each of the four core histones (H2A, H2B, H3 and H4) form the octamer, which is comprised of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Human Histone H3 is subject to trimethylation at Lys 9, a modification that may be necessary for select DNA transactions or chromatin state transitions. Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me). Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription. Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3).

Background References

1. Caeiro LD et al. Histone H3 mutations and their impact on genome stability maintenance. Biochem Soc Trans. 2024 Oct

2. Young D et al. The role of histone H3 lysine demethylases in glioblastoma. Cancer Metastasis Rev. 2023 Jun

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P68431 Human

SwissProt: P68433 Mouse

SwissProt: Q6LED0 Rat

Synonyms

H3 histone family member E pseudogene antibody

H3 histone family, member A antibody

H3/A antibody

H31_HUMAN antibody

H3F3 antibody

H3FA antibody

Hist1h3a antibody

HIST1H3B antibody

HIST1H3C antibody

HIST1H3D antibody

Expand

H3 histone family member E pseudogene antibody

H3 histone family, member A antibody

H3/A antibody

H31_HUMAN antibody

H3F3 antibody

H3FA antibody

Hist1h3a antibody

HIST1H3B antibody

HIST1H3C antibody

HIST1H3D antibody

HIST1H3E antibody

HIST1H3F antibody

HIST1H3G antibody

HIST1H3H antibody

HIST1H3I antibody

HIST1H3J antibody

HIST3H3 antibody

histone 1, H3a antibody

Histone cluster 1, H3a antibody

Histone H3 3 pseudogene antibody

Histone H3.1 antibody

Histone H3/a antibody

Histone H3/b antibody

Histone H3/c antibody

Histone H3/d antibody

Histone H3/f antibody

Histone H3/h antibody

Histone H3/i antibody

Histone H3/j antibody

Histone H3/k antibody

Histone H3/l antibody

Collapse

Images

Western blot analysis of Symmetric Di-Methyl-Histone H3 (R17) on different lysates with Rabbit anti-Symmetric Di-Methyl-Histone H3 (R17) antibody (HA724695) at 1/500 dilution.

Lane 1: 293T(Human embryonic kidney cell) cell lysate
Lane 2: NIH/3T3 (Mouse fibroblast) cell lysate
Lane 3: C6 (Rat glioma cell) cell lysate

Lysates/proteins at 20 µg/Lane.
Exposure time: 2 minutes; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724695, 1/500 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 15 kDa
Observed band size: 15 kDa
Application: Immunocytochemistry (IF-cell)

Species: Human
Sample: 293T (Human embryonic kidney cell)

Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.

Primary antibody: HA724695, 1/50, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).
Application: Immunocytochemistry (IF-cell)

Species: Mouse
Sample: NIH/3T3 (Mouse fibroblast)

Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.

Primary antibody: HA724695, 1/50, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).