Human/Monkey Albumin enzyme-linked immunoassay kit(one step)
Catalog# EHY0251
Human/Monkey Albumin enzyme-linked immunoassay kit(one step)
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ELISA
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Human
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15.63~1000 ng/ml
Overview
Product Name
Human/Monkey Albumin enzyme-linked immunoassay kit(one step)
Product Type
Elisa Kit
Sensitivity
0.19 ng/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0424 | 0.0450 | 0.0437 | |
| 15.63 | 0.1522 | 0.1529 | 0.1526 | 0.1093 |
| 31.25 | 0.2494 | 0.2556 | 0.2525 | 0.2092 |
| 62.50 | 0.4645 | 0.4753 | 0.4699 | 0.4266 |
| 125.00 | 0.8587 | 0.8714 | 0.8651 | 0.8218 |
| 250.00 | 1.5720 | 1.5830 | 1.5775 | 1.5342 |
| 500.00 | 2.6370 | 2.7020 | 2.6695 | 2.6262 |
| 1000.00 | 3.8760 | 3.9120 | 3.8940 | 3.8507 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 22.1 | 91.2 | 315.8 | 18.3 | 97.7 | 299.5 |
| Standard Deviation | 1.1 | 4.2 | 14.2 | 0.9 | 4.2 | 12.7 |
| Coefficient of Variation (%) | 5.0 | 4.7 | 4.5 | 4.8 | 4.3 | 4.2 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human/monkey Albumin into health human/monkey serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 107% with an overall mean recovery of 98%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (mg/ml) | Detectable (%) | Mean of Detectable (mg/ml) |
|---|---|---|---|---|
| Human Serum | 30 | 7.80-30.87 | 100 | 18.32 |
| Monkey Serum | 2 | 10.66-16.21 | 100 | 13.43 |
Serum/Plasma – Thirty samples from apparently healthy volunteers and two samples from healthy monkeys were evaluated for the presence of human/monkey Albumin in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃