Human/Monkey alpha Fetoprotein enzyme-linked immunoassay kit
Catalog# EH0203
Human/Monkey alpha Fetoprotein enzyme-linked immunoassay kit
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ELISA
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Human
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156.25~10000 pg/ml
Overview
Product Name
Human/Monkey alpha Fetoprotein enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
6.88 pg/ml (50 μl)；32.49 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0071 | 0.0068 | 0.0070 | |
| 156.25 | 0.0240 | 0.0229 | 0.0235 | 0.0165 |
| 312.50 | 0.0454 | 0.0476 | 0.0465 | 0.0396 |
| 625.00 | 0.1040 | 0.1062 | 0.1051 | 0.0982 |
| 1250.00 | 0.2739 | 0.2848 | 0.2794 | 0.2724 |
| 2500.00 | 0.7521 | 0.7844 | 0.7683 | 0.7613 |
| 5000.00 | 1.9240 | 1.9110 | 1.9175 | 1.9106 |
| 10000.00 | 3.6510 | 3.6680 | 3.6595 | 3.6526 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 184.3 | 899.0 | 2654.9 | 187.9 | 893.6 | 2794.1 |
| Standard Deviation | 14.1 | 56.6 | 92.1 | 6.7 | 37.5 | 36.0 |
| Coefficient of Variation (%) | 7.6 | 6.3 | 3.5 | 3.6 | 4.2 | 1.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human alpha Fetoprotein into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 86% to 110% with an overall mean recovery of 95%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Human Serum | 30 | 969.07-5411.95 | 100 | 2341.85 |
| Monkey Serum | 2 | 3165.04-3735.41 | 100 | 3450.22 |
Serum/Plasma – Thirty samples from apparently healthy volunteers and two samples from healthy monkeys were evaluated for the presence of human alpha Fetoprotein in this assay. No medical histories were available for the donors.
n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Afp
AFPD
Alpha fetoglobulin
Alpha fetoprotein
Alpha fetoprotein precursor
Alpha-1-fetoprotein
Alpha-fetoglobulin
Alpha-fetoprotein
alpha-fetoprotein, Hereditary persistence of, included
FETA
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