Human/Monkey CCL11/Eotaxin enzyme-linked immunoassay kit
Catalog# EH0201
Human/Monkey CCL11/Eotaxin enzyme-linked immunoassay kit
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ELISA
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Human
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62.5~4000 pg/ml
Overview
Product Name
Human/Monkey CCL11/Eotaxin enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
4.73 pg/ml (50 μl)；8.97 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0179 | 0.0182 | 0.0181 | |
| 62.50 | 0.0510 | 0.0524 | 0.0517 | 0.0337 |
| 125.00 | 0.0924 | 0.0940 | 0.0932 | 0.0752 |
| 250.00 | 0.1999 | 0.2008 | 0.2004 | 0.1823 |
| 500.00 | 0.4787 | 0.4712 | 0.4750 | 0.4569 |
| 1000.00 | 1.1480 | 1.1570 | 1.1525 | 1.1345 |
| 2000.00 | 2.7190 | 2.5540 | 2.6365 | 2.6185 |
| 4000.00 | 4.4816 | 4.4996 | 4.4906 | 4.4726 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 111.0 | 495.8 | 1676.5 | 100.0 | 402.4 | 1352.0 |
| Standard Deviation | 6.2 | 23.4 | 93.6 | 4.1 | 19.1 | 74.6 |
| Coefficient of Variation (%) | 5.6 | 4.7 | 5.6 | 4.1 | 4.7 | 5.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CCL11/Eotaxin into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 104% to 120% with an overall mean recovery of 117%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Human Serum | 30 | n.d.-151.13 | 57 | 38.40 |
| Monkey Serum | 2 | 171.40-175.20 | 100 | 173.30 |
Serum/Plasma – Thirty samples from apparently healthy volunteers and two samples from healthy monkeys were evaluated for the presence of human CCL11/Eotaxin in this assay. No medical histories were available for the donors.
n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
C-C motif chemokine 11
CCL 11
CCL11
CCL11_HUMAN
chemokine (C-C motif) ligand 11
Chemokine C C motif ligand 11
Chemokine CC Motif Ligand 11
Chemokine ligand 11
Eosinophil chemotactic protein
Eotaxin
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