Human/Monkey/Canine CXCL10/IP-10 enzyme-linked immunoassay kit
Catalog# EH0026
Human/Monkey/Canine CXCL10/IP-10 enzyme-linked immunoassay kit
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ELISA
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Human
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2.74~2000 pg/ml
Overview
Product Name
Human/Monkey/Canine CXCL10/IP-10 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.16 pg/ml (50 μl)；0.68 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0150 | 0.0145 | 0.0148 | |
| 2.74 | 0.0382 | 0.0344 | 0.0363 | 0.0216 |
| 8.23 | 0.0861 | 0.0827 | 0.0844 | 0.0697 |
| 24.69 | 0.2315 | 0.2262 | 0.2289 | 0.2141 |
| 74.07 | 0.6512 | 0.6404 | 0.6458 | 0.6311 |
| 222.22 | 1.8430 | 1.6300 | 1.7365 | 1.7218 |
| 666.67 | 3.1870 | 3.1500 | 3.1685 | 3.1538 |
| 2000.00 | 3.9030 | 3.8160 | 3.8595 | 3.8448 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 52.3 | 262.9 | 708.7 | 42.8 | 226.4 | 619.0 |
| Standard Deviation | 3.2 | 12.6 | 39.7 | 1.4 | 9.9 | 10.4 |
| Coefficient of Variation (%) | 6.1 | 4.8 | 5.6 | 3.3 | 4.4 | 1.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CXCL10 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 117% with an overall mean recovery of 100%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Human Serum | 30 | 8.36-99.65 | 100.0 | 25.02 |
| Monkey Serum | 2 | 101.58-109.84 | 100.0 | 105.71 |
| Canine Serum | 5 | 4.04-12.60 | 100.0 | 7.62 |
Serum/Plasma – Thirty samples from apparently healthy volunteers, two samples from healthy monkeys and five samples from healthy canines were evaluated for the presence of human CXCL10 in this assay. No medical histories were available for the donors.
n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Application in Publications
| ELISA | See 1 publications below |
Application in Publications
| Human | See 1 publications below |
Target
UNIPROT
Alias
Interferon gamma induced factor MOB1, mouse, homolog of
Interferon gamma induced protein 10
10 kDa interferon gamma induced protein
10 kDa interferon gamma-induced protein
C X C motif chemokine 10
C7
Chemokine (C X C motif) ligand 10
Chemokine CXC motif ligand 10
Crg 2
CRG2
ExpandFunction
Citation
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The cGAS-STING pathway activates transcription factor TFEB to stimulate lysosome biogenesis and pathogen clearance
Journal: Immunity
DOI:
IF: 25.5
Application: ELISA
Reactivity: Human
Publish date: 2024 Dec