Human/Monkey/Canine IL-18 enzyme-linked immunoassay kit
Catalog# EH0053
Human/Monkey/Canine IL-18 enzyme-linked immunoassay kit
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ELISA
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Human
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6.86~5000 pg/ml
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unconjugated
Overview
Product Name
Human/Monkey/Canine IL-18 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.56 pg/ml (50 μl)；1.28 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0449 | 0.0488 | 0.0469 | |
| 6.86 | 0.0752 | 0.0687 | 0.0720 | 0.0251 |
| 20.58 | 0.1038 | 0.0908 | 0.0973 | 0.0505 |
| 61.73 | 0.1749 | 0.1668 | 0.1709 | 0.1240 |
| 185.19 | 0.4421 | 0.4094 | 0.4258 | 0.3789 |
| 555.56 | 1.1370 | 1.1090 | 1.1230 | 1.0762 |
| 1666.67 | 2.7832 | 2.6478 | 2.7155 | 2.6687 |
| 5000.00 | 4.2824 | 4.3490 | 4.3157 | 4.2689 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 99.5 | 490.4 | 1512.9 | 101.2 | 481.1 | 1476.2 |
| Standard Deviation | 5.0 | 32.7 | 74.9 | 3.7 | 22.8 | 86.3 |
| Coefficient of Variation (%) | 5.1 | 6.7 | 4.9 | 3.6 | 4.7 | 5.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IL-18 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 84% to 111% with an overall mean recovery of 102%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Human Serum | 30 | 249.34-3143.95 | 100.0 | 1382.96 |
| Monkey Serum | 2 | 162.81-170.20 | 100.0 | 166.50 |
| Canine Serum | 5 | n.d.-5.71 | 60.0 | 4.44 |
Serum/Plasma – Thirty samples from apparently healthy volunteers, two samples from healthy monkeys and five samples from healthy canines were evaluated for the presence of human IL-18 in this assay. No medical histories were available for the donors.
n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Application in Publications
| ELISA | See 2 publications below |
Application in Publications
| Human | See 2 publications below |
Target
UNIPROT
Alias
Iboctadekin
IFN gamma inducing factor
IFN-gamma-inducing factor
IGIF
IL 1 gamma
IL 18
IL 1g
IL-1 gamma
IL-18
IL1 gamma
ExpandFunction
Citation
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A high-throughput reporter assay for screening potential gasdermin D inhibitors from natural products
Journal: International Journal Of Biological Macromolecules
DOI: 10.1016/j.ijbiomac.2026.150110
IF: 8.5
Application: ELISA
Reactivity: Human
Publish date: 2026 Jan
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Inhibition of the α7 nicotinic acetylcholine receptor alleviates pyroptosis in blood-brain barrier induced by Cryptococcus neoformans
Journal: Microbial Pathogenesis
DOI: 10.1016/j.micpath.2026.108377
IF: 3.5
Application: ELISA
Reactivity: Human
Publish date: 2026 Feb