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IL-6 Mouse Monoclonal Antibody [1-6]

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Catalog# EM1701-45

IL-6 Mouse Monoclonal Antibody [1-6]

Application

  • WB

  • IF-Cell

Reactivity

  • Mouse

  • Rat

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

IL-6 Mouse Monoclonal Antibody [1-6]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within Mouse IL-6 aa 1-211 / 211.

Species Reactivity

Mouse, Rat

Validated Applications

WB, IF-Cell

Molecular Weight

24 kDa

Positive Control

Recombinant protein lysates, EL4.

Conjugation

unconjugated

Clone Number

1-6

RRID

AB_3068769

Product Features

Form

Liquid

Concentration

2ug/ul

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG2b

Purification Method

Protein G affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IF-Cell

  • 1:50-1:1,000

Applications in Publications

WB See 15 publications below
IF See 2 publications below

Target

Function

Interleukin-6, or IL-6, is a multifunctional protein, 212 amino acids in length, that plays critical roles in host defense, immune response and hematopoiesis. IL-6 is constitutively expressed by epidermal Langerhans cells and its expression is induced in stimulated keratinocytes. IL-6, IL-1β and TNFα act as endogenous pyrogens, regulating the fever response to bacterial invasion. The IL-6 receptor is a trimeric complex composed of an IL-6-specific α chain and a homodimer of the gp130 glycoprotein common to the IL-6, IL-11, CNTF, OSM and LIF receptors. Stimulation with IL-6 leads to gp130 homodimerization and the activation of associated kinases JAK1 and JAK2. Once activated, JAK1 and JAK2 phosphorylate Stat3, causing its nuclear translocation and transcription of Stat3-responsive genes. IL-6 has also been shown to activate the Ras/MAP kinase pathway, which regulates NFIL6 transcription.

Background References

1. Luetticken C et al. Evidence for the importance of a positive charge and an alpha-helical structure of the C-terminus for biological activity of human IL-6. FEBS Lett 282:265-267 (1991).

2. van Damme J et al. Separation and comparison of two monokines with lymphocyte-activating factor activity: IL-1 beta and hybridoma growth factor (HGF). Identification of leukocyte-derived HGF as IL-6. J Immunol 140:1534-1541 (1988).

Sequence Similarity

Belongs to the IL-6 superfamily.

Post-translational Modification

N- and O-glycosylated.

Subcellular Location

Secreted.

UNIPROT

SwissProt: P08505 Mouse

Synonyms

Interleukin BSF 2 antibody

B cell differentiation factor antibody

B cell stimulatory factor 2 antibody

B-cell stimulatory factor 2 antibody

BSF 2 antibody

BSF-2 antibody

BSF2 antibody

CDF antibody

CTL differentiation factor antibody

Hepatocyte stimulatory factor antibody

Expand

Images

  • Western blot analysis of IL6 on recombinant protein lysate using anti-IL6 antibody at 1/1,000 dilution.

    Western blot analysis of IL6 on recombinant protein lysate using anti-IL6 antibody at 1/1,000 dilution.

  • Immunocytochemistry analysis of EL4 cells labeling IL-6 with Mouse anti-IL-6 antibody (<a href="/products/EM1701-45" style="font-weight: bold;text-decoration: underline;">EM1701-45</a>) at 1/1,000 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-IL-6 antibody (<a href="/products/EM1701-45" style="font-weight: bold;text-decoration: underline;">EM1701-45</a>) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />beta Tubulin (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 594, <a href="/products/HA1122" style="font-weight: bold;text-decoration: underline;">HA1122</a>) were used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of EL4 cells labeling IL-6 with Mouse anti-IL-6 antibody (EM1701-45) at 1/1,000 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-IL-6 antibody (EM1701-45) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Filter:
  1. WB
  2. IF

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