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PSD95 Recombinant Rabbit Monoclonal Antibody [SR38-09]

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Catalog# ET1602-20

PSD95 Recombinant Rabbit Monoclonal Antibody [SR38-09]

Application

  • WB

  • IF-Tissue

  • IHC-P

  • IP

  • IHC-Fr

Reactivity

  • Human

  • Mouse

  • Rat

Predicted reactivity

  • Cynomolgus monkey

  • Pig

Predicted species support after-sales service
This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

PSD95 Recombinant Rabbit Monoclonal Antibody [SR38-09]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human PSD95 aa 1-40.

Species Reactivity

Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)

Predicted species support after-sales service

Validated Applications

WB, IF-Tissue, IHC-P, IP, IHC-Fr

Molecular Weight

Predicted band size: 80 kDa

Positive Control

Human brain tissue lysate, mouse brain tissue lysate, mouse hippocampus tissue lysate, rat brain tissue lysate, rat hippocampus tissue lysate, mouse brain tissue, mouse retina tissue, rat brain tissue, rat retina tissue, mouse cerebellum tissue.

Conjugation

unconjugated

Clone Number

SR38-09

RRID

AB_3069633

Product Features

Form

Liquid

Concentration

1ug/ul

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IF-Tissue

  • 1:500

  • IHC-P

  • 1:2,000

  • IP

  • Use at an assay dependent concentration.

  • IHC-Fr

  • 1:500-1:1,000

Species in Publications

Mouse See 6 publications below
Rat See 4 publications below

Target

Function

PSD-95 (postsynaptic density protein 95) also known as SAP-90 (synapse-associated protein 90) is a protein that in humans is encoded by the DLG4 (discs large homolog 4) gene. PSD-95 is a member of the membrane-associated guanylate kinase (MAGUK) family. With PSD-93 it is recruited into the same NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. PSD-95 is the best studied member of the MAGUK-family of PDZ domain-containing proteins. Like all MAGUK-family proteins, its basic structure includes three PDZ domains, an SH3 domain, and a guanylate kinase-like domain (GK) connected by disordered linker regions. It is almost exclusively located in the post synaptic density of neurons, and is involved in anchoring synaptic proteins. Its direct and indirect binding partners include neuroligin, NMDA receptors, AMPA receptors, and potassium channels. It plays an important role in synaptic plasticity and the stabilization of synaptic changes during long-term potentiation.

Background References

1. Planagumà J et al. Human N-methyl D-aspartate receptor antibodies alter memory and behaviour in mice. Brain 138:94-109 (2015).

2. Zhou L et al. Neonatal exposure to sevoflurane may not cause learning and memory deficits and behavioral abnormality in the childhood of Cynomolgus monkeys. Sci Rep 5:11145 (2015).

Sequence Similarity

Belongs to the MAGUK family.

Tissue Specificity

Brain.

Post-translational Modification

Palmitoylated. Palmitoylation is required for targeting to postsynaptic density, plasma membrane and synapses (By similarity). Palmitoylation by ZDHHC2 occurs when the synaptic activity decreases and induces DLG4 synaptic clustering (By similarity). Palmitoylation may play a role in glutamate receptor GRIA1 synapse clustering (By similarity). Depalmitoylated by ABHD17A and ABHD17B and to a lesser extent by ABHD17C, ABHD12, ABHD13, LYPLA1 and LYPLA2. Undergoes rapid synaptic palmitoylation/depalmitoylation cycles during neuronal development which slow down in mature neurons (By similarity).; Ubiquitinated by MDM2 in response to NMDA receptor activation, leading to proteasome-mediated degradation of DLG4 which is required for AMPA receptor endocytosis.

Subcellular Location

Cell membrane, Postsynaptic density, Synapse, Cytoplasm, Cell projection, axon, dendritic spine, dendrite, Presynapse.

UNIPROT

SwissProt: P78352 Human

SwissProt: Q62108 Mouse

SwissProt: P31016 Rat

Synonyms

Discs large homolog 4 antibody

Disks large homolog 4 antibody

DLG 4 antibody

Dlg4 antibody

DLG4_HUMAN antibody

FLJ97752 antibody

FLJ98574 antibody

Human post synaptic density protein 95 antibody

Post synaptic density protein 95 antibody

Postsynaptic density protein 95 antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebellum <br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1: 500 (PSD95, <a href="/products/ET1602-20" style="font-weight: bold;text-decoration: underline;">ET1602-20</a>, red); 1:500 (Iba1, <a href="/products/HA601376" style="font-weight: bold;text-decoration: underline;">HA601376</a>, green)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1: 500 (PSD95, ET1602-20, red); 1:500 (Iba1, HA601376, green)

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Hippocampus<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1: 1,000 (PSD95, <a href="/products/ET1602-20" style="font-weight: bold;text-decoration: underline;">ET1602-20</a>, red); 1:500 (VGLUT2, <a href="/products/HA601393" style="font-weight: bold;text-decoration: underline;">HA601393</a>, green)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Hippocampus

    Sample: Frozen section

    Antibody concentration: 1: 1,000 (PSD95, ET1602-20, red); 1:500 (VGLUT2, HA601393, green)

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: Cerebellum <br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1: 1,000 (PSD95, <a href="/products/ET1602-20" style="font-weight: bold;text-decoration: underline;">ET1602-20</a>, red); 1:500 (VGLUT2, <a href="/products/HA601393" style="font-weight: bold;text-decoration: underline;">HA601393</a>, green)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Rat

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1: 1,000 (PSD95, ET1602-20, red); 1:500 (VGLUT2, HA601393, green)

    Antigen retrieval: Not required

  • Application: IF-tissue<br /><br />Species: Mouse<br /><br />Site: Cerebellum<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1:500

    Application: IF-tissue

    Species: Mouse

    Site: Cerebellum

    Sample: Paraffin-embedded section

    Antibody concentration: 1:500

  • Western blot analysis of PSD95 on different lysates with Rabbit anti-PSD95 antibody (<a href="/products/ET1602-20" style="font-weight: bold;text-decoration: underline;">ET1602-20</a>) at 1/2,000 dilution.<br /><br />Lane 1: Human brain tissue lysate (20 µg/Lane)<br />Lane 2: Mouse brain tissue lysate (20 µg/Lane)<br />Lane 3: Mouse hippocampus tissue lysate (20 µg/Lane)<br />Lane 4: Rat brain tissue lysate (20 µg/Lane)<br />Lane 5: Rat hippocampus tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 80 kDa<br />Observed band size: 75-100 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1602-20" style="font-weight: bold;text-decoration: underline;">ET1602-20</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PSD95 on different lysates with Rabbit anti-PSD95 antibody (ET1602-20) at 1/2,000 dilution.

    Lane 1: Human brain tissue lysate (20 µg/Lane)
    Lane 2: Mouse brain tissue lysate (20 µg/Lane)
    Lane 3: Mouse hippocampus tissue lysate (20 µg/Lane)
    Lane 4: Rat brain tissue lysate (20 µg/Lane)
    Lane 5: Rat hippocampus tissue lysate (20 µg/Lane)

    Predicted band size: 80 kDa
    Observed band size: 75-100 kDa

    Exposure time: 20 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-20) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-PSD95 antibody (<a href="/products/ET1602-20" style="font-weight: bold;text-decoration: underline;">ET1602-20</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1602-20" style="font-weight: bold;text-decoration: underline;">ET1602-20</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-PSD95 antibody (ET1602-20) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-20) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-PSD95 antibody (<a href="/products/ET1602-20" style="font-weight: bold;text-decoration: underline;">ET1602-20</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1602-20" style="font-weight: bold;text-decoration: underline;">ET1602-20</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-PSD95 antibody (ET1602-20) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-20) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PSD95 antibody (<a href="/products/ET1602-20" style="font-weight: bold;text-decoration: underline;">ET1602-20</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1602-20" style="font-weight: bold;text-decoration: underline;">ET1602-20</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PSD95 antibody (ET1602-20) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-20) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse retina tissue with Rabbit anti-PSD95 antibody (<a href="/products/ET1602-20" style="font-weight: bold;text-decoration: underline;">ET1602-20</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1602-20" style="font-weight: bold;text-decoration: underline;">ET1602-20</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse retina tissue with Rabbit anti-PSD95 antibody (ET1602-20) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-20) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-PSD95 antibody (<a href="/products/ET1602-20" style="font-weight: bold;text-decoration: underline;">ET1602-20</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1602-20" style="font-weight: bold;text-decoration: underline;">ET1602-20</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-PSD95 antibody (ET1602-20) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-20) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat retina tissue with Rabbit anti-PSD95 antibody (<a href="/products/ET1602-20" style="font-weight: bold;text-decoration: underline;">ET1602-20</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1602-20" style="font-weight: bold;text-decoration: underline;">ET1602-20</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat retina tissue with Rabbit anti-PSD95 antibody (ET1602-20) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-20) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Filter:
  1. WB
  2. IF
  3. Co-IP

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