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Iba1 Recombinant Antibody [JM36-62] - Chicken IgY (Chimeric)

Specification

Quota

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Contact: Sanry CHEN

Email: info@bio-station.net

Phone: 852-35650223

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Catalog# HA601376

Iba1 Recombinant Antibody [JM36-62] - Chicken IgY (Chimeric)

Application

  • IHC-Fr

  • WB

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Predicted reactivity

  • Pig

  • Cynomolgus monkey

Predicted species support after-sales service

Overview

Product Name

Iba1 Recombinant Antibody [JM36-62] - Chicken IgY (Chimeric)

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Synthetic peptide within N-terminal human Iba1.

Product Specificity

This Chicken Recombinant monoclonal chimeric antibody has been engineered from a HiMab parent antibody (ET1705-78). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed FC-reactive secondary antibodies are recommended.

Species Reactivity

Human, Mouse, Rat (Predicted: Pig, Cynomolgus monkey)

Predicted species support after-sales service

Validated Applications

IHC-Fr, WB, FC

Molecular Weight

Predicted band size: 17 kDa

Positive Control

Mouse cerebellum tissue, mouse spleen tissue, THP-1 cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, THP-1.

Conjugation

unconjugated

Clone Number

JM36-62

Product Features

Form

Liquid

Concentration

1ug/ul

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgY

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-Fr

  • 1:500

  • WB

  • 1:5,000

  • FC

  • 1:1,000

Target

Function

Ionized calcium-binding adapter molecule 1 (Iba1), also known as allograft inflammatory factor-1 (AIF-1), is a 147 amino acid cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. Iba1, containing two EF domains, is induced by cytokines and interferons. In an unstimulated state, Iba1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding Iba1 maps to chromosome 6p21.33 and resides in the tumor necrosis factor (TNF) cluster of genes located in the region represented by the human major histocompatibility complex (MHC).

Background References

1. Hennessy E et al. Systemic TNF-a produces acute cognitive dysfunction and exaggerated sickness behavior when superimposed upon progressive neurodegeneration. Brain Behav Immun 59:233-244 (2017).

2. Arentsen T et al. The bacterial peptidoglycan-sensing molecule Pglyrp2 modulates brain development and behavior. Mol Psychiatry 22:257-266 (2017).

Subcellular Location

Cytoplasm, cytoskeleton, Cell projection, ruffle membrane, Cell projection, phagocytic cup.

UNIPROT

SwissProt: P55008 Human

SwissProt: O70200 Mouse

SwissProt: P55009 Rat

Synonyms

AIF 1 antibody

AIF-1 antibody

Aif1 antibody

AIF1 protein antibody

AIF1_HUMAN antibody

Allograft inflammatory factor 1 antibody

Allograft inflammatory factor 1 splice variant G antibody

allograft inflammatory factor-1 splice variant Hara-1 antibody

balloon angioplasty responsive transcription antibody

BART 1 antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebellum<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500 (Iba1, <a href="/products/HA601376" style="font-weight: bold;text-decoration: underline;">HA601376</a>, green); 1/500 (MAG, <a href="/products/HA721818" style="font-weight: bold;text-decoration: underline;">HA721818</a>, red)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1/500 (Iba1, HA601376, green); 1/500 (MAG, HA721818, red)

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebellum<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500 (Iba1, <a href="/products/HA601376" style="font-weight: bold;text-decoration: underline;">HA601376</a>, green); 1/500 (PSD95, <a href="/products/ET1602-20" style="font-weight: bold;text-decoration: underline;">ET1602-20</a>, red)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1/500 (Iba1, HA601376, green); 1/500 (PSD95, ET1602-20, red)

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Spleen<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500 (Iba1, <a href="/products/HA601376" style="font-weight: bold;text-decoration: underline;">HA601376</a>, green); 1/1,000 (F4/80, <a href="/products/HA721520" style="font-weight: bold;text-decoration: underline;">HA721520</a>, red)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Spleen

    Sample: Frozen section

    Antibody concentration: 1/500 (Iba1, HA601376, green); 1/1,000 (F4/80, HA721520, red)

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: Spleen<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500 (Iba1, <a href="/products/HA601376" style="font-weight: bold;text-decoration: underline;">HA601376</a>, green); 1/1,000 (F4/80, <a href="/products/HA721520" style="font-weight: bold;text-decoration: underline;">HA721520</a>, red)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Rat

    Site: Spleen

    Sample: Frozen section

    Antibody concentration: 1/500 (Iba1, HA601376, green); 1/1,000 (F4/80, HA721520, red)

    Antigen retrieval: Not required

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Iba1 on different lysates with Chicken anti-Iba1 antibody (<a href="/products/HA601376" style="font-weight: bold;text-decoration: underline;">HA601376</a>) at 1/5,000 dilution.<br /><br />Lane 1: THP-1 cell lysate<br />Lane 2: HEK-293 cell lysate (negative)<br />Lane 3: Mouse spleen tissue lysate<br />Lane 4: Rat spleen tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 17 kDa<br />Observed band size: 17 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601376" style="font-weight: bold;text-decoration: underline;">HA601376</a>) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat anti-Chicken IgY - HRP Secondary Antibody (HA1146) at 1/10,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of Iba1 on different lysates with Chicken anti-Iba1 antibody (HA601376) at 1/5,000 dilution.

    Lane 1: THP-1 cell lysate
    Lane 2: HEK-293 cell lysate (negative)
    Lane 3: Mouse spleen tissue lysate
    Lane 4: Rat spleen tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 17 kDa
    Observed band size: 17 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601376) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat anti-Chicken IgY - HRP Secondary Antibody (HA1146) at 1/10,000 dilution was used for 1 hour at room temperature.

  • Flow cytometric analysis of THP-1 cells labeling Iba1.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA601376" style="font-weight: bold;text-decoration: underline;">HA601376</a>, 1/1,000) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 750 conjugate-Goat anti-Chicken IgY Secondary antibody (HA1145) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of THP-1 cells labeling Iba1.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA601376, 1/1,000) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 750 conjugate-Goat anti-Chicken IgY Secondary antibody (HA1145) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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